headshot of Thomas Smithgall

Thomas Smithgall



Emert-Sedlak, L.A., Tice, C.M., Shi, H., Alvarado, J.J., Shu, S.T., Reitz, A.B., & Smithgall, T.E. (2024). PROTAC-mediated degradation of HIV-1 Nef efficiently restores cell-surface CD4 and MHC-I expression and blocks HIV-1 replication. Cell Chem Biol, 31(4), 658-668.e14.Elsevier BV. doi: 10.1016/j.chembiol.2024.02.004.

Heinrich, F., Thomas, C.E., Alvarado, J.J., Eells, R., Thomas, A., Doucet, M., Whitlatch, K.N., Aryal, M., Losche, M., & Smithgall, T.E. (2023). Neutron Reflectometry and Molecular Simulations Demonstrate HIV-1 Nef Homodimer Formation on Model Lipid Bilayers. JOURNAL OF MOLECULAR BIOLOGY, 435(8), 168009.Elsevier BV. doi: 10.1016/j.jmb.2023.168009.

Du, S., Alvarado, J.J., Wales, T.E., Moroco, J.A., Engen, J.R., & Smithgall, T.E. (2022). ATP-site inhibitors induce unique conformations of the acute myeloid leukemia-associated Src-family kinase, Fgr. STRUCTURE, 30(11), 1508-+.Elsevier BV. doi: 10.1016/j.str.2022.08.008.

Fai, L.W., Aryal, M., Shu, S.T., & Smithgall, T.E. (2020). HIV-1 Nef dimers short-circuit immune receptor signaling by activating Tec-family kinases at the host cell membrane. JOURNAL OF BIOLOGICAL CHEMISTRY, 295(15), 5163-5174.Elsevier BV. doi: 10.1074/jbc.RA120.012536.

Staudt, R.P., & Smithgall, T.E. (2020). Nef homodimers down-regulate SERINC5 by AP-2-mediated endocytosis to promote HIV-1 infectivity. JOURNAL OF BIOLOGICAL CHEMISTRY, 295(46), 15540-15552.Elsevier BV. doi: 10.1074/jbc.RA120.014668.

Staudt, R.P., Alvarado, J.J., Emert-Sedlak, L.A., Shi, H., Shu, S.T., Wales, T.E., Engen, J.R., & Smithgall, T.E. (2020). Structure, function, and inhibitor targeting of HIV-1 Nef-effector kinase complexes. JOURNAL OF BIOLOGICAL CHEMISTRY, 295(44), 15158-15171.Elsevier BV. doi: 10.1074/jbc.REV120.012317.

Moroco, J.A., Alvarado, J.J., Staudt, R.P., Shi, H., Wales, T.E., Smithgall, T.E., & Engen, J.R. (2018). Remodeling of HIV-1 Nef Structure by Src-Family Kinase Binding. JOURNAL OF MOLECULAR BIOLOGY, 430(3), 310-321.Elsevier BV. doi: 10.1016/j.jmb.2017.12.008.

Shen, K., Moroco, J.A., Patel, R.K., Shi, H., Engen, J.R., Dorman, H.R., & Smithgall, T.E. (2018). The Src family kinase Fgr is a transforming oncoprotein that functions independently of SH3-SH2 domain regulation. SCIENCE SIGNALING, 11(553).American Association for the Advancement of Science (AAAS). doi: 10.1126/scisignal.aat5916.

Shu, S.T., Emert-Sedlak, L.A., & Smithgall, T.E. (2017). Cell-based Fluorescence Complementation Reveals a Role for HIV-1 Nef Protein Dimerization in AP-2 Adaptor Recruitment and CD4 Co-receptor Down-regulation. JOURNAL OF BIOLOGICAL CHEMISTRY, 292(7), 2670-2678.Elsevier BV. doi: 10.1074/jbc.M116.770016.

Emert-Sedlak, L.A., Loughran, H.M., Shi, H., III, K.J.L., Shu, S.T., Zhao, J., Day, B.W., Wrobel, J.E., Reitz, A.B., & Smithgall, T.E. (2016). Synthesis and evaluation of orally active small molecule HIV-1 Nef antagonists. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 26(5), 1480-1484.Elsevier BV. doi: 10.1016/j.bmcl.2016.01.043.

Grover, P., Shi, H., Baumgartner, M., Camacho, C.J., & Smithgall, T.E. (2015). Fluorescence Polarization Screening Assays for Small Molecule Allosteric Modulators of ABL Kinase Function. In Buday, L. (Ed.). PLOS ONE, 10(7), e0133590.Public Library of Science (PLoS). doi: 10.1371/journal.pone.0133590.

Alvarado, J.J., Tarafdar, S., Yeh, J.I., & Smithgall, T.E. (2014). Interaction with the Src Homology (SH3-SH2) Region of the Src-family Kinase Hck Structures the HIV-1 Nef Dimer for Kinase Activation and Effector Recruitment. JOURNAL OF BIOLOGICAL CHEMISTRY, 289(41), 28539-28553.Elsevier BV. doi: 10.1074/jbc.M114.600031.

Iyer, P.C., Zhao, J., Emert-Sedlak, L.A., Moore, K.K., Smithgall, T.E., & Day, B.W. (2014). Synthesis and structure-activity analysis of diphenylpyrazolodiazene inhibitors of the HIV-1 Nef virulence factor. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 24(7), 1702-1706.Elsevier BV. doi: 10.1016/j.bmcl.2014.02.045.

Moroco, J.A., Craigo, J.K., Iacob, R.E., Wales, T.E., Engen, J.R., & Smithgall, T.E. (2014). Differential Sensitivity of Src-Family Kinases to Activation by SH3 Domain Displacement. In Sticht, H. (Ed.). PLOS ONE, 9(8), e105629.Public Library of Science (PLoS). doi: 10.1371/journal.pone.0105629.

Tarafdar, S., Poe, J.A., & Smithgall, T.E. (2014). The Accessory Factor Nef Links HIV-1 to Tec/Btk Kinases in an Src Homology 3 Domain-dependent Manner. JOURNAL OF BIOLOGICAL CHEMISTRY, 289(22), 15718-15728.Elsevier BV. doi: 10.1074/jbc.M114.572099.

Zhang, X., Simerly, C., Hartnett, C., Schatten, G., & Smithgall, T.E. (2014). Src-family tyrosine kinase activities are essential for differentiation of human embryonic stem cells. STEM CELL RESEARCH, 13(3), 379-389.Elsevier BV. doi: 10.1016/j.scr.2014.09.007.

Perrone, R., Nadai, M., Poe, J.A., Frasson, I., Palumbo, M., Palu, G., Smithgall, T.E., & Richter, S.N. (2013). Formation of a Unique Cluster of G-Quadruplex Structures in the HIV-1 nef Coding Region: Implications for Antiviral Activity. PLOS ONE, 8(8), e73121.Public Library of Science (PLoS). doi: 10.1371/journal.pone.0073121.

Trible, R.P., Narute, P., Emert-Sedlak, L.A., Alvarado, J.J., Atkins, K., Thomas, L., Kodama, T., Yanamala, N., Korotchenko, V., Day, B.W., Thomas, G., & Smithgall, T.E. (2013). Discovery of a diaminoquinoxaline benzenesulfonamide antagonist of HIV-1 Nef function using a yeast-based phenotypic screen. RETROVIROLOGY, 10(1), 135.Springer Science and Business Media LLC. doi: 10.1186/1742-4690-10-135.

Narute, P.S., & Smithgall, T.E. (2012). Nef Alleles from All Major HIV-1 Clades Activate Src-Family Kinases and Enhance HIV-1 Replication in an Inhibitor-Sensitive Manner. In Vartanian, J.P. (Ed.). PLOS ONE, 7(2), e32561.Public Library of Science (PLoS). doi: 10.1371/journal.pone.0032561.

Pene-Dumitrescu, T., Shu, S.T., Wales, T.E., Alvarado, J.J., Shi, H., Narute, P., Moroco, J.A., Yeh, J.I., Engen, J.R., & Smithgall, T.E. (2012). HIV-1 Nef interaction influences the ATP-binding site of the Src-family kinase, Hck. BMC Chem Biol, 12(1), 1.Springer Science and Business Media LLC. doi: 10.1186/1472-6769-12-1.

O'Reilly, L.P., Watkins, S.C., & Smithgall, T.E. (2011). An Unexpected Role for the Clock Protein Timeless in Developmental Apoptosis. PLOS ONE, 6(2), e17157.Public Library of Science (PLoS). doi: 10.1371/journal.pone.0017157.