Pitt | Swanson Engineering
Publications
  1. Allen A., Gau D., Francoeur P., Sturm J., Wang Y., Martin R., Maranchie J., Duensing A., Kaczorowski A., Duensing S., Wu L., Lotze MT., Koes D., Storkus WJ., Roy P. (2020) Actin-binding protein profilin1 promotes aggressiveness of clear-cell renal cell carcinoma cells. J. Biol Chem Nov;295(46):15636-15649.
  2. Gau D., Vignaud L., Allen A., Guo Z., Sahel J., Boone D., Koes D., Guillonneau X., Roy P. (2020) Disruption of profilin1 function suppresses developmental and pathological retinal neovascularization. J. Biol Chem Jul;295(28):9618-9629.
  3. Gau D., Veon W., Shroff S., Roy P. (2019) The VASP-profilin1 (Pfn1) interaction is critical for efficient cell migration and is regulated by cell-substrate adhesion in a PKA-dependent manner J. Biol Chem Feb 27. pii:jbc.RA118.005255.
  4. Chakraborty S., Jiang C., Gau D., Oddo M., Ding Z., Vollmer L., Joy M., Scheimann W., Stolz D., Vogt A., Ghosh S., Roy P. (2018) Profilin-1 deficiency leads to SMAD3 upregulation and impaired 3D outgrowth of breast cancer cells British Journal of Cancer Oct;119(9):1106-1117.
  5. Gau D., Roy P. (2018) SRF'ing and SAP'ing: role of MRTF proteins in cell migration J. Cell Science Oct 11;131(19). pii: jcs218222.
  6. Gau D., Lewis T., McDermott L., Wipf P., Koes D., Roy P. (2018) Structure-based virtual screening identifies small molecule inhibitor of the profilin-actin interaction J. Biol Chem  Feb 16;293(7):2606-2616.
  7. Joy M, Gau D, Castellucci N, Prywes R., Roy P (2017) The Myocardin-related transcription factor MKL co-regulates the levels of two profilin isoforms J. Biol Chem 292(28):11777-11791. 
  8. Gau D., Veon W., Capasso T, Bottcher R., Shroff S., Roman B., Roy P. (2017) Pharmacological intervention of MKL/SRF signaling by CCG-1423 impedes endothelial cell migration and angiogenesis; Angiogenesis 20(4):663-672.
  9. Jiang C, Ding Z., Joy M., Chakraborty S., Kim S., Bottcher R., Condeelis J., Singh S., Roy P. (2017) A balanced level of profilin-1 promotes stemness and tumor-initiating potential of breast cancer cells Cell Cycle 16(24):2366-2373.
  10. Ding Z. Joy M., Kameneva M., Roy P (2017): Nanomolar concentration of blood-soluble drag-reducing polymer inhibits experimental metastasis of human breast cancer cells: Breast Cancer: Targets and Therapy: 9:61-65.
  11. Gau D., Veon W., Koes D., Zeng X., Yates N., Shroff S., Roy P (2016) Threonine 89 is an important residue of profilin-1 that is phosphorylatable by protein-kinase A PLoS One. May 26;11(5):e0156313.
  12. Jiang C., Veon W., Li H, Hallows K., Roy P. (2015) Epithelial morphological reversion drives Profilin-1-induced elevation of p27kip1 in mesenchymal triple-negative human breast cancer cells through AMP-activated Kinase activation Cell Cycle 15:1-10.
  13. Gau D., Lesnock J. , Hood B., Bhargava R., Sun M., Darcy K., Conrads T., Edwards R., Kelley J., Krivak T., Roy P. (2015)  BRCA1 deficiency in ovarian cancer is associated with alteration in expression of several key regulators of cell motility – A proteomics study.  Cell Cycle 14(12):1884-92.
  14. Valenzuela-Iglesias A., Sharma V., Beaty B., Ding Z., Gutierrez-Millan L., Roy P., Condeelis J., Bravo-Cordero  J. (2015) Profilin1 regulates invadopodium maturation in human breast cáncer cells Eur. J. Cell Biol 94(2):78-89.
  15. Ding Z., Joy M.., Bhargava R., Gunsaulus M., Lakshman N., Miron-Mendoza M.,  Petroll M., Condeelis J., Wells A., Roy P. (2014) Profilin-1 downregulation has contrasting effects on early vs late steps of breast cancer metastasis Oncogene 33(16):2065-74.
  16. Coumans J., Gau D., Poljak A., Wasinger V., Roy P., Moens P. (2014) Profilin-1 overexpression in MDA-MB-231 breast cancer cells is associated with alteration in proteomic markers of cell proliferation, survival and motility as revealed by global proteomics analyses OMICS- A Journal of Integrative Biology. 18(12):778-91
  17. Joy M., Vollmer L., Hulkower K., Peterson C., Dutch Boltz R., Stern A., Roy P*., Vogt V. (2014) A high-content, multiplexed screen in human breast cancer cells identifies profilin-1 inducers with anti-migratory activities PLoS One 9(2):e88350. doi: 10.1371/journal.pone. 0088350. [* - co-corresponding author]
  18. Coumans J., Gau D., Poljak A., Wasinger V., Roy P., Moens P. (2014) Green fluorescent protein expression triggers proteome changes in breast cancer cells Exp Cell Res. 320(1):33-45.
  19. Ding Z. Bae YH, and Roy. P (2012). Molecular insights on context-specific role of profilin-1 in cell migration. Cell Adhesion and Migration 6(5): 442-449 PMID: 23076048.
  20. Gau D., Ding Z., Baty C., Roy P.  (2011)Fluorescence resonance energy transfer based interaction between VASP and profilin Cellular and Molecular Bioengineering 4(1):1-8.
  21. Bae Y., Ding Z., Das T. Wells A., Gertler F., Roy P. (2010): Profilin1 regulates PI(3,4)P2 and lamellipodin accumulation at the leading edge thus influencing motility of MDA-MB-231 cells PNAS 107(50): 21547-21552
  22. Leloup L., Hanshuang S.,  Bae Y., Deasy B., Stolz D., Roy P., Wells  A (2010)  m-Calpain activation is regulated by its membrane localization and by its binding to phosphatidylinositol 4,5-bisphosphate. J. Biol Chem 285(43):33549-66
  23. Zou L., Ding Z., Roy P. (2010): Profilin-1 overexpression inhibits proliferation of MDA-MB-231 breast cancer cells through p27kip1 upregulation J. Cell Physiology 223(3):623-629. PMCID: PMC2872929
  24. Kam L., Roy P. (2010): Cell mechanics and signaling: From micro to nano. Cellular and Molecular Bioengineering 3(1):1-2.
  25. Das T., Bae Y, Wells A., and Roy P. (2009): Profilin-1 overexpression upregulates PTEN and suppresses AKT activation in breast cancer cells. J. Cell Physiology 218(2): 436-443. PMCID: PMC2874249­
  26. Bae Y., Ding Z., Zou L., Wells A., Gertler F., Roy P. (2009): Loss of Profilin-1 expression enhances breast cancer cell motility by Ena/VASP proteins J. Cell Physiology 219(2):354-364. PMCID: PMC2990882
  27. Zou L., Hazan R., Roy P. (2009): Profilin-1 overexpression restores adherence junctions in MDA-MB-231 cells in R-cadherin dependent manner Cell motility and Cytoskeleton 68: 1048-1056. PMCID: PMC2783708
  28. Ding Z., Gau D., Deasy B, Wells A., Roy P. (2009): Both actin and polyproline interactions of profilin-1 are required for vascular endothelial cell migration, invasion and capillary morphogenesis Exp Cell Res 315 (17): 2963-2973. PMCID: PMC2757537.

  • Zou L., Jaramillo M., Whaley D., Wells A., Panchapakesa V., Das T., Roy P. (2007): Profilin-1 is a negative regulator of mammary carcinoma aggressiveness. British J. of Cancer 97: 1361-1371. PMCID: PMC2360229
  • Humphrey D, Rajfur Z, Imperiali B, Marriott G, Roy P, Jacobson K (2007). Design, synthesis, and characterization of caged compounds. CSH protocols. 2007; 2007:pdb.ip25.
  • Humphrey D, Rajfur Z, Imperiali B, Marriott G, Roy P, Jacobson K (2007) Introduction of caged peptide/protein into cells using bead loading. CSH protocols 2007:pdb.prot4658.
  • Humphrey D, Rajfur Z, Imperiali B, Marriott G, Roy P, Jacobson K. (2007) Introduction of caged peptide/protein into cells using microinjection  . CSH protocols. 2007:pdb.prot4659.
  • Ding Z., Lambrechts A., Parepally M., Roy P. (2006): Silencing profilin-1 inhibits endothelial cell proliferation, migration and cord morphogenesis. J. Cell Science 119: 4127-4137.
  • Boukhelifa M., Moza M., Johansson J., Rachlin A., Parast M., Huttelmaier S., Roy P., Jockusch B. M., Carpen O., Karlsson R., Otey C. A. (2006): The proline-rich protein palladin is a binding partner for profilin. FEBS J. 273(1) 26-33.
  • Roy P., Jacobson K. (2004): Overexpression of profilin reduces the migration of invasive breast cancer cells. Cell Motility and Cytoskeleton 57(2): 84-95.
  • Marriott, G., Jacobson K., Roy, P. (2003): Preparation and light-directed activation of caged proteins. Methods in Enzymol. (Biophotonics) 360:274-288.
  • Roy P., Rajfur Z., Pomorski P., Jacobson K. (2002): Microscope-based techniques for studying cell adhesion and migration. Nature Cell Biology 4(4): E91-96.
  • Rajfur Z., Roy P., Otey C., Romer L., Jacobson K. (2002): Dissecting the link between stress fibers and focal adhesions by CALI of EGFP-fusion proteins. Nature Cell Biology 4(4): 286-293.
  • Roy P., Rajfur Z., Jones D., Marriott G., Loew L., Jacobson K. (2001): Local photorelease of caged-Tb4 in locomoting keratocytes causes cell turning. J of Cell Biology 153 (5): 1035-1048.
  • Mar P. K.  Roy P., Yin H. L., Cavanagh H. D., Jester J. V. (2001): Stress fiber formation is required for matrix reorganization in a corneal myofibroblast cell line. Experimental Eye Research 72(4): 455-66.
  • Roy P., Petroll W. M., Chuong C. J., Cavanagh H. D., Jester J. V.  (1999): Effect of cell migration on the maintenance of tension on a collagen matrix. Annals of Biomedical Engg. 27(6): 721-730.
  • Roy P., Petroll W. M., Cavanagh H. D., Jester J. V. (1999): Exertion of Tractional Force Requires the Coordinated Up-Regulation of Cell Contractility and Adhesion. Cell Motility and Cytoskeleton 42: 23-34
  • Roy P., Petroll W. M., Cavanagh H. D., Chuong C. J., Jester J. V. (1997): An in vitro force measurement assay to study the early mechanical interaction between corneal fibroblasts and collagen matrix. Experimental Cell Research 232(1): 106-117.
  • Petroll W. M., Roy P., Chuong C. J.,  Hall B.,  Cavanagh H. D., Jester J. V.  (1996): Measurement of surgically induced corneal deformations using three-dimensional confocal microscopy. Cornea 15(2): 154-164.
  • Roy P., Petroll W. M., McKinney A. E., Chuong C. J. (1996): Computational models of the effects of hydration on corneal biomechanics and the results of Radial Keratotomy. J. Biomech. Engg. (Trans. of ASME). 118: 255-258.
  • Roy P., Pal S. (1992): A computer-aided graphics interactive system for geometric parameter analysis of complex biological sections. Automedica 15: 47-56.
  • Radhakrishnan S., Roy P., Ganesan N (1992): A comparative evaluation of different treatment methods of scoliosis correction by finite element technique. Automedica 13: 253-266.