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Mohammad Ataai

Professor
Chem/Petroleum Engineering

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Ph.D., Chemical Engineering, Cornell University, 1986

M.S., Chemical Engineering, Tufts University, 1981

Meade, J., Bartlow, P., Trivedi, R.N., Akhtar, P., Ataai, M.M., Khan, S.A., & Domach, M.M. (2015). Effect of plasmid replication deregulation via inc mutations on E-coli proteome & simple flux model analysis. MICROBIAL CELL FACTORIES, 14(1), 31.Springer Science and Business Media LLC. doi: 10.1186/s12934-015-0212-x.

Haley, R., Fruchtl, M., Brune, E.M., Ataai, M., Henry, R., & Beitle, R. (2014). A redesigned Escherichia coli triosephosphate isomerase restores growth properties in a bacterial strain useful for Immobilized Metal Affinity Chromatography (IMAC). JOURNAL OF BIOTECHNOLOGY, 188, 48-52.Elsevier BV. doi: 10.1016/j.jbiotec.2014.07.432.

Trivedi, R.N., Akhtar, P., Meade, J., Bartlow, P., Ataai, M.M., Khan, S.A., & Domach, M.M. (2014). High-Level Production of Plasmid DNA by Escherichia coli DH5 alpha Omega sacB by Introducing inc Mutations. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(23), 7154-7160.American Society for Microbiology. doi: 10.1128/AEM.02445-14.

Bartlow, P., Tiwari, N., Beitle, R.R., & Ataai, M.M. (2012). Evaluation of Escherichia coli proteins that burden nonaffinity-based chromatography as a potential strategy for improved purification performance. BIOTECHNOLOGY PROGRESS, 28(1), 137-145.Wiley. doi: 10.1002/btpr.703.

Meade, J., Khan, S., Ataai, M., & Domach, M. (2012). Use of flux pre-analysis to enable C-13 tracer studies in pyruvate kinase-deficient Escherichia coli. BIOTECHNOLOGY JOURNAL, 7(3), 449-462.Wiley. doi: 10.1002/biot.201100338.

Bartlow, P., Uechi, G.T., Jr, C.J.J., Sultana, T., Fruchtl, M., Beitle, R.R., & Ataai, M.M. (2011). Identification of native Escherichia coli BL21 (DE3) proteins that bind to immobilized metal affinity chromatography under high imidazole conditions and use of 2D-DIGE to evaluate contamination pools with respect to recombinant protein expression level. PROTEIN EXPRESSION AND PURIFICATION, 78(2), 216-224.Elsevier BV. doi: 10.1016/j.pep.2011.04.021.

Pan, Z., Cunningham, D.S., Zhu, T., Ye, K., Koepsel, R.R., Domach, M.M., & Ataai, M.M. (2010). Enhanced recombinant protein production in pyruvate kinase mutant of Bacillus subtilis. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 85(6), 1769-1778.Springer Science and Business Media LLC. doi: 10.1007/s00253-009-2244-9.

Tiwari, N., Woods, L., Haley, R., Kight, A., Goforth, R., Clark, K., Ataai, M., Henry, R., & Beitle, R. (2010). Identification and characterization of native proteins of Escherichia coli BL-21 that display affinity towards Immobilized Metal Affinity Chromatography and Hydrophobic Interaction Chromatography Matrices. PROTEIN EXPRESSION AND PURIFICATION, 70(2), 191-195.Elsevier BV. doi: 10.1016/j.pep.2009.10.018.

Cunningham, D.S., Koepsel, R.R., Ataai, M.M., & Domach, M.M. (2009). Factors affecting plasmid production in Escherichia coli from a resource allocation standpoint. MICROBIAL CELL FACTORIES, 8(1), 27.Springer Science and Business Media LLC. doi: 10.1186/1475-2859-8-27.

Cunningham, D.S., Liu, Z., Domagalski, N., Koepsel, R.R., Ataai, M.M., & Domach, M.M. (2009). Pyruvate Kinase-Deficient Escherichia coli Exhibits Increased Plasmid Copy Number and Cyclic AMP Levels. JOURNAL OF BACTERIOLOGY, 191(9), 3041-3049.American Society for Microbiology. doi: 10.1128/JB.01422-08.

Dilmore, R., Griffith, C., Liu, Z., Soong, Y., Hedges, S.W., Koepsel, R., & Ataai, M. (2009). Carbonic anhydrase-facilitated CO2 absorption with polyacrylamide buffering bead capture. INTERNATIONAL JOURNAL OF GREENHOUSE GAS CONTROL, 3(4), 401-410.Elsevier BV. doi: 10.1016/j.ijggc.2009.01.004.

Grant, K.G., Krisky, D.M., Ataai, M.M., & III, G.J.C. (2009). Engineering Cell Lines for Production of Replication Defective HSV-1 Gene Therapy Vectors. BIOTECHNOLOGY AND BIOENGINEERING, 102(4), 1087-1097.Wiley. doi: 10.1002/bit.22123.

Liu, Z., Bartlow, P., Dilmore, R.M., Soong, Y., Pan, Z., Koepsel, R., & Ataai, M. (2009). Production, Purification, and Characterization of a Fusion Protein of Carbonic Anhydrase from Neisseria gonorrhoeae and Cellulose Binding Domain from Clostridium thermocellum. BIOTECHNOLOGY PROGRESS, 25(1), 68-74.Wiley. doi: 10.1002/btpr.80.

Liu, Z., Bartlow, P., Varakata, R., Beitle, R., Koepsel, R., & Ataa, M.M. (2009). Use of proteomics for design of a tailored host cell for highly efficient protein purification. JOURNAL OF CHROMATOGRAPHY A, 1216(12), 2433-2438.Elsevier BV. doi: 10.1016/j.chroma.2009.01.020.

Ghosh, S., Grossmann, I.E., Ataai, M.M., & Domach, M.M. (2006). A three-level problem-centric strategy for selecting NMR precursor labeling and analytes. METABOLIC ENGINEERING, 8(5), 491-507.Elsevier BV. doi: 10.1016/j.ymben.2006.05.001.

Jiang, C., Ataai, M., Ozuer, A., Krisky, D., Wechuck, J., Pornsuwan, S., Pourarian, F., & Glorioso, J.C. (2006). Inactivation of herpes simplex type 1 gene vector on immobilized metal affinity chromatography: Oxidative damage by hydroxyl free radicals and its prevention. BIOTECHNOLOGY AND BIOENGINEERING, 95(1), 48-57.Wiley. doi: 10.1002/bit.20943.

Jiang, C., Gloioso, J.C., & Ataai, M.M. (2006). Presence of Imidazole in Loading Buffer Prevents Formation of Free Radical in IMAC and Dramatically Improves the Recovery of HSV-1 Gene Therapy Vectors. J. Chromatography A, J Chromatogr A, 1121(1), 40-45.

Jiang, C., Glorioso, J.C., & Ataai, M. (2006). Presence of imidazole in loading buffer prevents formation of free radical in immobilized metal affinity chromatography and dramatically improves the recovery of herpes simplex virus type 1 gene therapy vectors. JOURNAL OF CHROMATOGRAPHY A, 1121(1), 40-45.Elsevier BV. doi: 10.1016/j.chroma.2006.04.071.

Pan, Z., Zhu, T., Domagalski, N., Khan, S., Koepsel, R.R., Domach, M.M., & Ataai, M.M. (2006). Regulating expression of pyruvate kinase in Bacillus subtilis for control of growth rate and formation of acidic byproducts. BIOTECHNOLOGY PROGRESS, 22(5), 1451-1455.Wiley. doi: 10.1021/bp060049u.

Pan, Z., Zhu, T., Domagalski, N., Khan, S., Koepsel, R.R., Domach, M.M., & Ataai, M.M. (2006). Regulating expression of pyruvate kinase in Bacillus subtilis for control of growth rate and formation of acidic byproducts. Biotechnol Prog, 22(5), 1451-1455. doi: 10.1021/bp060049u.

Ghosh, S., Zhu, T., Grossmann, I.E., Ataai, M.M., & Domach, M.M. (2005). Closing the loop between feasible flux scenario identification for construct evaluation and resolution of realized fluxes via NMR. COMPUTERS & CHEMICAL ENGINEERING, 29(3), 459-466.Elsevier BV. doi: 10.1016/j.compchemeng.2004.08.027.

Zhu, T., Pan, Z., Domagalski, N., Koepsel, R., Ataai, M.M., & Domach, M.M. (2005). Engineering of Bacillus subtilis for enhanced total synthesis of folic acid. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(11), 7122-7129.American Society for Microbiology. doi: 10.1128/AEM.71.11.7122-7129.2005.

Jiang, C.P., Wechuck, J.B., Goins, W.F., Krisky, D.M., Wolfe, D., Ataai, M.M., & Glorioso, J.C. (2004). Immobilized cobalt affinity chromatography provides a novel, efficient method for herpes simplex virus type 1 gene vector purification. JOURNAL OF VIROLOGY, 78(17), 8994-9006.American Society for Microbiology. doi: 10.1128/JVI.78.17.8994-9006.2004.

Ye, K., Jin, S., Ataai, M.M., & Schultz, S. (2004). Tagging retrovirus with a metal binding peptide: a novel approach for preparation of clinical-grade retroviral vectors by immobilized metal affinity chromatography purification. J. Virol., 78, 9820-9827.

Ye, K.M., Jin, S., Ataai, M.M., Schultz, J.S., & Ibeh, J. (2004). Tagging retrovirus vectors with a metal binding peptide and one-step purification by immobilized metal affinity chromatography. JOURNAL OF VIROLOGY, 78(18), 9820-9827.American Society for Microbiology. doi: 10.1128/JVI.78.18.9820-9827.2004.

Zhu, T., Ataai, M.M., Domach, M.M., & Grossmann, I.E. (2003). A metabolic network analysis & NMR experimental design program with user interface-driven model construction. Metabolic Engineering, 5, 74-85.

Zhu, T., Grossmann, I.E., Domach, M.M., & Ataai, M.M. (2003). Theoretical improvement of folic acid production in E. coli. Fermentation Biotechnology, ACS symposium series, 862, 207-219.

Zhu, T., Phalakornkule, C., Ghosh, S., Grossmann, I.E., Koepsel, R.R., Ataai, M.M., & Domach, M.M. (2003). A metabolic network analysis & NMR experiment design tool with user interface-driven model construction for depth-first search analysis. METABOLIC ENGINEERING, 5(2), 74-85.Elsevier BV. doi: 10.1016/S1096-7176(03)00023-5.

Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (2002). Flux regulation patterns and energy audit of E-coli B/r and K-12. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 12(2), 258-267.

Ozuer, A., Wechuck, J.B., Russell, B., Wolfe, D., Goins, W.F., Glorioso, J.C., & Ataai, M.M. (2002). Evaluation of Infection Parameters in the Production of HSV-1 Viral Vectors For Gene Therapy. Biotech. Prog., 18, 476-482.

Ozuer, A., Wechuck, J.B., Russell, B., Wolfe, D., Goins, W.F., Glorioso, J.C., & Ataai, M.M. (2002). Evaluation of infection parameters in the production of replication-defective HSV-1 viral vectors. BIOTECHNOLOGY PROGRESS, 18(3), 476-482.Wiley. doi: 10.1021/bp010176k.

Ozuer, A., Wetchuck, J.C., Goins, W.F., Wolfe, D., Glorioso, J.C., & Ataai, M.M. (2002). Effect of Genetic Background, Culture Conditions, and Reactor Configuration on Productivity of HSV-1 Vectors. Biotechnol. Bioeng., 77, 685-692.

Wechuck, J.B., Ozuer, A., Goins, W.F., Wolfe, D., Oligino, T., Glorioso, J.C., & Ataai, M.M. (2002). Effect of temperature, medium composition, and cell passage on production of herpes-based viral vectors. BIOTECHNOLOGY AND BIOENGINEERING, 79(1), 112-119.Wiley. doi: 10.1002/bit.10310.

Phalakornkule, C., Lee, S., Zhu, T., Koepsel, R., Ataai, M.M., Grossmann, I.E., & Domach, M.M. (2001). A MILP-based flux alternative generation and NMR experimental design strategy for metabolic engineering. METABOLIC ENGINEERING, 3(2), 124-137.Elsevier BV. doi: 10.1006/mben.2000.0165.

Ward, M.S., Ataai, M., Koepsel, R.R., & Shepherd, R.E. (2001). Comparison of energy-minimized structures of [Pd-II(N-methyliminodiacetate)] complexes of X-1-His-X-3-His-His peptides as an analysis of steric and specific interactions with synthetic binding tags for IMAC separations. BIOTECHNOLOGY PROGRESS, 17(4), 712-719.Wiley. doi: 10.1021/bp010050n.

Zhu, T., Phalakornkule, C., Koepsel, R.R., Domach, M.M., & Ataai, M.M. (2001). Cell growth and by-product formation in a pyruvate kinase mutant of E-coli. BIOTECHNOLOGY PROGRESS, 17(4), 624-628.Wiley. doi: 10.1021/bp0100575.

Chen, Y., Pasquinelli, R., Ataai, M., Koepsel, R.R., Kortes, R.A., & Shepherd, R.E. (2000). Coordination of two high-affinity hexamer peptides to copper(II) and palladium(II) models of the peptide-metal chelation site on IMAC resins. INORGANIC CHEMISTRY, 39(6), 1180-1186.American Chemical Society (ACS). doi: 10.1021/ic990520z.

Fry, B., Zhu, T., Domach, M.M., Koepsel, R.R., Phalakornkule, C., & Ataai, M.M. (2000). Characterization of growth and acid formation in a Bacillus subtilis pyruvate kinase mutant. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66(9), 4045-4049.American Society for Microbiology. doi: 10.1128/AEM.66.9.4045-4049.2000.

Fry, B., Zhu, T., Phalakornkule, C., Koepsel, R., Domach, M.M., & Ataai, M.M. (2000). Characterization of Growth and Acid Formation in a Bacillus subtilis Pyruvate Kinase mutant. Appl Environ. Microbiol, 66, 1045-1049.

Lee, S., Phalakornkule, C., Ataai, M.M., Domach, M.M., & Grossmann, I.E. (2000). Recursive MILP Model for Finding All the Alternate Optima in LP Models for Metabolic Networks. Computers in Chemical Engineering, 39, 1180-1186.

Pasquinelli, R.S., Shepherd, R.E., Koepsel, R.R., Zhao, A., & Ataai, M.M. (2000). Design of affinity tags for one-step protein purification from immobilized zinc columns. BIOTECHNOLOGY PROGRESS, 16(1), 86-91.Wiley. doi: 10.1021/bp990139h.

Phalakornkule, C., Fry, B., Zhu, T., Kopesel, R., Ataai, M.M., & Domach, M.M. (2000). 13C NMR evidence for pyruvate kinase flux attenuation underlying suppressed acid formation in Bacillus subtilis. BIOTECHNOLOGY PROGRESS, 16(2), 169-175.Wiley. doi: 10.1021/bp000007k.

Wechuck, J.B., Goins, W.F., Glorioso, J.C., & Ataai, M.M. (2000). Effect of protease inhibitors on yield of HSV-1-based viral vectors. BIOTECHNOLOGY PROGRESS, 16(3), 493-496.Wiley. doi: 10.1021/bp000016t.

Wu, N.X., & Ataai, M.M. (2000). Production of viral vectors for gene therapy applications. CURRENT OPINION IN BIOTECHNOLOGY, 11(2), 205-208.Elsevier BV. doi: 10.1016/S0958-1669(00)00080-X.

Chen, Y., Pasquinelli, R., Ataai, M., Koepsel, R.R., Stringfield, T.W., & Shepherd, R.E. (1999). Coordination of the six-mer peptide Gly-His-Pro-His-His-Gly to Cu-II and (PdN)-N-II-methyliminodiacetate complexes as IMAC chelation site models. JOURNAL OF INORGANIC BIOCHEMISTRY, 76(3-4), 211-220.Elsevier BV. doi: 10.1016/S0162-0134(99)00129-4.

Goel, A., Lee, J., Domach, M.M., & Ataai, M.M. (1999). Metabolic fluxes, pools, and enzyme measurements suggest a tighter coupling of energetics and biosynthetic reactions associated with reduced pyruvate kinase flux. BIOTECHNOLOGY AND BIOENGINEERING, 64(2), 129-134.Wiley. doi: 10.1002/(SICI)1097-0290(19990720)64:2<129::AID-BIT1>3.0.CO;2-I.

Goel, A., Lee, J., Domach, M.M., & Ataai, M.M. (1999). Metabolic fluxes, pools, and enzyme measurements suggest a tighter coupling of energetics and biosynthetic reactions associated with reduced pyruvate kinase flux. Biotechnology and Bioengineering, 64(2), 129-134. doi: 10.1002/(SICI)1097-0290(19990720)64:2<129::AID-BIT1>3.0.CO;2-I.

Pasquinelli, R.S., Koepsel, R.R., Wu, N.X., & Ataai, M.M. (1999). Vector engineering anomalies: Impact on fusion protein purification performance. PROTEIN EXPRESSION AND PURIFICATION, 17(3), 449-455.Elsevier BV. doi: 10.1006/prep.1999.1152.

Owen, M.J., & Karsenty, G. (1998). New developments in bone formation. 7, 363-366.Ovid Technologies (Wolters Kluwer Health). doi: 10.1097/00041552-199807000-00003.

Patwardhan, A., Pascquinelli, R., Koepsel, R., & Ataai, M.M. (1998). Phage-displayed libraries for the selection of optimal affinity tails for Ni-NTA purification of recombinant proteins. Biotechnology Techniques, 12, 421-424.

Patwardhan, A.V., Goud, G.N., Pasquinelli, R.S., Koepsel, R.R., & Ataai, M.M. (1998). Phage-displayed libraries for the selection of optimal affinity peptides for protein purification using Ni-nitrilotriacetic Acid chromatography. BIOTECHNOLOGY TECHNIQUES, 12(6), 421-424.Springer Science and Business Media LLC. doi: 10.1023/A:1008842902587.

Bhatia, R., Jesionowski, G., Ferrance, J., & Ataai, M.M. (1997). Insect cell physiology. CYTOTECHNOLOGY, 24(1), 1-9. doi: 10.1023/B:CYTO.0000010410.24541.dd.

Bhatia, R., Jesionowski, G., Ferrance, J., & Ataai, M.M. (1997). Insect cell physiology. Cytotechnology, 24(1), 1-9.Springer Science and Business Media LLC. doi: 10.1023/b:cyto.0000010410.24541.dd.

Bhatia, R., Jesionowski, G., Ferrance, J., & Ataai, M.M. (1997). Erratum: Insect cell physiology (Cytotechnology (1996) 20 (33-41)). Cytotechnology, 24(1), 87.

Goud, G.N., Patwardhan, A.V., Beckman, E.J., Ataai, M.M., & Koepsel, R.R. (1997). Selection of specific peptide ligands for immobilized metals using a phage displayed library: Application to protein separation using IMAC. International Journal of Bio-Chromatography, 3(2), 123-136.

Goud, N., Patwardhan, A., Koepsel, R., & Ataai, M.M. (1997). Selection of specific peptide ligands for Immobilized Cu (II) using a phage displayed peptide library on bacteriophage. Intl. J. Biochromatography, 3, 123-136.

Jesionowski, G., & Ataai, M.M. (1997). Drastic improvements in insect cell/reccombinat baculovirus protein productivity cultured in a physiologically designed medium. Biotechnology Progress, 13, 355-360.

Jesionowski, G.A., & Ataai, M.M. (1997). An efficient medium for high protein production in the insect cell baculovirus expression system. BIOTECHNOLOGY PROGRESS, 13(4), 355-360.Wiley. doi: 10.1021/bp970054l.

Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (1997). optimality of co-feeding of carbon sources for maximizing cellular and energetic yields using a constrained network analysis. Appl Environ. Microbiol., 63, 710-718.

Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (1997). Supply-side analysis of growth of Bacillus subtilis on glucose-citrate medium: Feasible network alternatives and yield optimality. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 63(2), 710-718.American Society for Microbiology. doi: 10.1128/AEM.63.2.710-718.1997.

Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (1997). Supply-Side Analysis of Growth of Bacillus subtilis on Glucose-Citrate Medium: Feasible Network Alternatives and Yield Optimality. Applied and environmental microbiology, 63(5), 2109.American Society for Microbiology. doi: 10.1128/aem.63.5.2109-2109.1997.

Patwardhan, A.V., & Ataai, M.M. (1997). Site accessibility and the pH dependence of the saturation capacity of a highly cross-linked matrix - Immobilized metal affinity chromatography of bovine serum albumin on Chelating Superose. JOURNAL OF CHROMATOGRAPHY A, 767(1-2), 11-23.Elsevier BV. doi: 10.1016/S0021-9673(97)00013-7.

Patwardhan, A.V., & Ataai, M.M. (1997). Mathematical modeling of protein purification of E. coli cell extract using immobilized metal affinity chromatography: Effect of loading pH on the column capacity and purity of the target protein. AIChE J, 43, 3232-3240.

Patwardhan, A.V., & Ataai, M.M. (1997). IMA chromatography of a cell extract: Effect of loading pH on protein purification. AICHE JOURNAL, 43(12), 3232-3240.Wiley. doi: 10.1002/aic.690431211.

Patwardhan, A.V., Goud, G.N., Koepsel, R.R., & Ataai, M.M. (1997). Selection of optimum affinity tags from a phage-displayed peptide library - Application to immobilized copper(II) affinity chromatography. JOURNAL OF CHROMATOGRAPHY A, 787(1-2), 91-100.Elsevier BV. doi: 10.1016/S0021-9673(97)00580-3.

Wagner, W.R., Muzzio, D.J., Rilo, H.R., Deglau, T., Ataai, M.M., Michalopoulos, G.K., & Block, G.D. (1997). Effect of growth factors and defined medium on primary hepatocyte culture on polyester carriers with varying surface treatment. TISSUE ENGINEERING, 3(3), 289-301.Mary Ann Liebert Inc. doi: 10.1089/ten.1997.3.289.

Wagner, W.R., Muzzio, D.J., Rilo, H.R., Deglaus, T., & Ataai, M.M. (1997). Effect of Growth Factors and Defined Medium on Primary Hepatocyte Culture on Polyester Carriers With Varying Surface Treatments. Tissue Engineering, 3, 298-301.

Bhatia, R., Jesionowski, G., Ferrance, J., & Ataai, M.M. (1996). Insect cell physiology. 20, (pp. 33-41).Springer Science and Business Media LLC. doi: 10.1007/BF00350387.

Goel, A., Domach, M.M., Hanley, W., Lee, J.W., & Ataai, M.M. (1996). Coordination of glycolysis and TCA cycle reaction networks - Citrate-glucose cometabolism eliminates acids and reveals potential metabolic engineering strategies. RECOMBINANT DNA BIOTECHNOLOGY III: THE INTEGRATION OF BIOLOGICAL AND ENGINEERING SCIENCES, 782(1), 1-16.Wiley. doi: 10.1111/j.1749-6632.1996.tb40542.x.

Hauck, J.T., Olson, G.J., Scierka, S.J., Perry, M.B., & Ataai, M.M. (1996). Effects of simulated flue gas on growth of microalgae. ACS Division of Fuel Chemistry, Preprints, 41(4), 1391-1394.

Parwardhan, A.V., & Ataai, M.M. (1996). One step purification of human interleukin 1b using IMAC. J. Biochromatography, 2, 119-124.

Patwardhan, A.V., & Ataai, M.M. (1996). One step purification of human interleukin-1β from the cellular protein of Escherichia coli using the immobilized metal affinity chromatography. International Journal of Bio-Chromatography, 2(2), 119-124.

Patwardhan, A.V., Ataai, M.M., & Zenouzi, M. (1996). Mathematical modeling and optimization of the immobilized metal affinity chromatography. American Society of Mechanical Engineers, Bioengineering Division (Publication) BED, 33, 223-224.

GOEL, A., LEE, J., DOMACH, M.M., & ATAAI, M.M. (1995). SUPPRESSED ACID FORMATION BY COFEEDING OF GLUCOSE AND CITRATE IN BACILLUS CULTURES - EMERGENCE OF PYRUVATE-KINASE AS A POTENTIAL METABOLIC ENGINEERING SITE. BIOTECHNOLOGY PROGRESS, 11(4), 380-385.Wiley. doi: 10.1021/bp00034a003.

Parwardhan, A.V., Zenouzi, M., & Ataai, M.M. (1995). Design of efficient loading and elution strategies for IMAC separations: purification of human interleukin 1B from E. coli cell extract. AIChE Proceedings On Recent Development and Future Opportunities In Separation Technology, 1, 418-424.

Patwardhan, A.V., Ataai, M.M., & Zenouzi, M. (1995). Mathematical model of the immobilized metal affinity chromatography for protein separations: Simulation of loading and elution conditions. American Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD, 322, 61-68.

VIERHELLER, C., GOEL, A., PETERSON, M., DOMACH, M.M., & ATAAI, M.M. (1995). SUSTAINED AND CONSTITUTIVE HIGH-LEVELS OF PROTEIN-PRODUCTION IN CONTINUOUS CULTURES OF BACILLUS-SUBTILIS. BIOTECHNOLOGY AND BIOENGINEERING, 47(5), 520-524.Wiley. doi: 10.1002/bit.260470503.

Lee, J., Goel, A., Ataai, M.M., & Domach, M.M. (1994). Flux adaptation of a citrate synthase mutant of E. coli. Ann. NY. Acad. Sci., 745, 35-59.

LEE, J., GOEL, A., ATAAI, M.M., & DOMACH, M.M. (1994). FLUX ADAPTATIONS OF CITRATE SYNTHASE DEFICIENT ESCHERICHIA-COLI. BIOCHEMICAL ENGINEERING VIII, 745(1), 35-50.Wiley. doi: 10.1111/j.1749-6632.1994.tb44362.x.

ALMEIDA, N.F., BECKMAN, E.J., & ATAAI, M.M. (1993). IMMOBILIZATION OF GLUCOSE-OXIDASE IN THIN POLYPYRROLE FILMS - INFLUENCE OF POLYMERIZATION CONDITIONS AND FILM THICKNESS ON THE ACTIVITY AND STABILITY OF THE IMMOBILIZED ENZYME. BIOTECHNOLOGY AND BIOENGINEERING, 42(9), 1037-1045.Wiley. doi: 10.1002/bit.260420904.

Beitle, R.R., & Ataai, M.M. (1993). An effective, modular interfacing network for the monitoring of culture parameters: Acquisition of data from several RS-232 devices. Process Control and Quality, 4(2), 99-107.

BEITLE, R.R., & ATAAI, M.M. (1993). ONE-STEP PURIFICATION OF A MODEL PERIPLASMIC PROTEIN FROM INCLUSION-BODIES BY ITS FUSION TO AN EFFECTIVE METAL-BINDING PEPTIDE. BIOTECHNOLOGY PROGRESS, 9(1), 64-69.Wiley. doi: 10.1021/bp00019a009.

Beitle, R.R., Sagar, S.L., Ataai, M.M., & Domach, M.M. (1993). Metal-based affinity separation of a- and b-chymotrypsin. Bioseparation, 3, 291-296.

FERRANCE, J.P., GOEL, A., & ATAAI, M.M. (1993). UTILIZATION OF GLUCOSE AND AMINO-ACIDS IN INSECT-CELL CULTURES - QUANTIFYING THE METABOLIC FLOWS WITHIN THE PRIMARY PATHWAYS AND MEDIUM DEVELOPMENT. BIOTECHNOLOGY AND BIOENGINEERING, 42(6), 697-707.Wiley. doi: 10.1002/bit.260420604.

GOEL, A., FERRANCE, J., JEONG, J., & ATAAI, M.M. (1993). ANALYSIS OF METABOLIC FLUXES IN BATCH AND CONTINUOUS CULTURES OF BACILLUS-SUBTILIS. BIOTECHNOLOGY AND BIOENGINEERING, 42(6), 686-696.Wiley. doi: 10.1002/bit.260420603.

Sagar, S.L., Beitle, R.R., Ataai, M.M., & Domach, M.M. (1992). Metal-based affinity separation of alpha- and gamma-chymotrypsin and thermal stability analysis of isolates. Bioseparation, 3(5), 291-296.

BEITLE, R.R., & ATAAI, M.M. (1991). THE PURIFICATION OF TEM-BETA-LACTAMASE FROM CELL EXTRACT USING IMMOBILIZED COPPER AFFINITY-CHROMATOGRAPHY. BIOTECHNOLOGY TECHNIQUES, 5(2), 107-110.Springer Science and Business Media LLC. doi: 10.1007/BF00159980.

BEITLE, R.R., & ATAAI, M.M. (1991). A UNIQUE MONITORING AND CONTROL-SYSTEM FOR LABORATORY FERMENTERS. BIOTECHNOLOGY TECHNIQUES, 5(2), 77-82.Springer Science and Business Media LLC. doi: 10.1007/BF00159974.

JEONG, J.W., & ATAAI, M.M. (1990). A HIGHLY STRUCTURED MODEL FOR SIMULATION OF BATCH AND CONTINUOUS CULTURES OF BACILLUS-SUBTILIS AND EXAMINATION OF CELLULAR-DIFFERENTIATION. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 589(1 Biochemical E), 82-90.Wiley. doi: 10.1111/j.1749-6632.1990.tb24236.x.

Jeong, J.W., Snay, J., & Ataai, M.M. (1990). A Model For Examining Growth and Sporulation Processes of B. subtilis. Biotechnol. Bioeng., 35, 160-184.

JEONG, J.W., SNAY, J., & ATAAI, M.M. (1990). A MATHEMATICAL-MODEL FOR EXAMINING GROWTH AND SPORULATION PROCESSES OF BACILLUS-SUBTILIS. BIOTECHNOLOGY AND BIOENGINEERING, 35(2), 160-184.Wiley. doi: 10.1002/bit.260350208.

SNAY, J., JEONG, J.W., & ATAAI, M.M. (1989). EFFECTS OF GROWTH-CONDITIONS ON CARBON UTILIZATION AND ORGANIC BY-PRODUCT FORMATION IN B-SUBTILIS. BIOTECHNOLOGY PROGRESS, 5(2), 63-69.Wiley. doi: 10.1002/btpr.5420050207.

Longo, A., Snay, J., Zimmer, L., & Ataai, M.M. (1988). Calcium Dependence of Proteolytic Activity of Residual Protease of B. subtilis. Biotechnology Letters, 10, 649-654.

LONGO, A., SNAY, J.R., ZIMMER, L., & ATAAI, M.M. (1988). CALCIUM REQUIREMENTS OF RESIDUAL PROTEASE IN BACILLUS-SUBTILIS DB104. BIOTECHNOLOGY LETTERS, 10(9), 649-654.Springer Science and Business Media LLC. doi: 10.1007/BF01024719.

Ataai, M.M., & Shuler, M.L. (1987). A Mathematical Model for Prediction of Plasmid Copy Number and Genetic Stability in E. coli,. Biotechnol. Bioeng., 30(389-397).

ATAAI, M.M., & SHULER, M.L. (1987). A MATHEMATICAL-MODEL FOR PREDICTION OF PLASMID COPY NUMBER AND GENETIC STABILITY IN ESCHERICHIA-COLI. BIOTECHNOLOGY AND BIOENGINEERING, 30(3), 389-397.Wiley. doi: 10.1002/bit.260300310.

KIM, B.G., GOOD, T.A., ATAAI, M.M., & SHULER, M.L. (1987). GROWTH-BEHAVIOR AND PREDICTION OF COPY NUMBER AND RETENTION OF COLE1-TYPE PLASMIDS IN ESCHERICHIA-COLI UNDER SLOW GROWTH-CONDITIONS. ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 506(1 Biochemical E), 384-395.Wiley. doi: 10.1111/j.1749-6632.1987.tb23835.x.

ATAAI, M.M., & SHULER, M.L. (1986). MATHEMATICAL-MODEL FOR THE CONTROL OF COLE1 TYPE PLASMID REPLICATION. PLASMID, 16(3), 204-212.Elsevier BV. doi: 10.1016/0147-619X(86)90058-2.

ATAAI, M.M., & SHULER, M.L. (1985). SIMULATION OF THE GROWTH-PATTERN OF A SINGLE CELL OF ESCHERICHIA-COLI UNDER ANAEROBIC CONDITIONS. BIOTECHNOLOGY AND BIOENGINEERING, 27(7), 1027-1035.Wiley. doi: 10.1002/bit.260270714.

ATAAI, M.M., & SHULER, M.L. (1985). SIMULATION OF CFSTR THROUGH DEVELOPMENT OF A MATHEMATICAL-MODEL FOR ANAEROBIC GROWTH OF ESCHERICHIA-COLI CELL-POPULATION. BIOTECHNOLOGY AND BIOENGINEERING, 27(7), 1051-1055.Wiley. doi: 10.1002/bit.260270717.

LEE, A.L., ATAAI, M.M., & SHULER, M.L. (1984). DOUBLE-SUBSTRATE-LIMITED GROWTH OF ESCHERICHIA-COLI. BIOTECHNOLOGY AND BIOENGINEERING, 26(11), 1398-1401.Wiley. doi: 10.1002/bit.260261120.

FROHLICH, B.T., WEBSTER, I.A., ATAAI, M.M., & SHULER, M.L. (1983). PHOTOBIOREACTORS - MODELS FOR INTERACTION OF LIGHT-INTENSITY, REACTOR DESIGN, AND ALGAL PHYSIOLOGY. BIOTECHNOLOGY AND BIOENGINEERING, 13(13), 331-350.

Liu, Z., Bartlow, P., Dilmore, R.M., Soong, Y., Pan, Z., Koepsel, R., & Ataai, M. (2009). Production, purification, and characterization of a fusion protein of carbonic anhydrase from Neisseria gonorrhoeae and cellulose binding domain from Clostridium thermocellum. AIChE mtg.United States. doi: 10.1002/btpr.80.

Grant, K.G., Krisky, D.M., Ataai, M.M., & III, G.J.C. (2006). Engineering Cell Lines for the Production of Highly Replication Defective HSV-1 Vectors. International Herpesvirus Workshop.Asheville, NC. doi: 10.1016/j.ymthe.2006.08.1216.

Domagalski, N.R., Pan, Z.W., Zhu, T., Ye, K.M., Koepsel, R., Ataai, M.M., & Domach, M.M. (2005). Combining enhanced traits of E-coli into B-subtilis. ACS Natl Mtg.San Diego, CA.

Grant, K.G., Ataai, M.M., Glorioso, J.C., & Krisky, D. (2005). Cell line development for production of clinical grade HSV vectors. ACS Natl Mtg.San Diego, CA.

Haley, R., Henry, R., Goforth, R., Ataai, M.M., & Beitle, R.R. (2005). Circumventing the effects of high binding immobilized metal affinity chromatography contaminants. AICHE 2005 annual meeting.Cincinnati, OH.

Jiang, C., Wechuck, J., Ozuer, A., Glorioso, J., & Ataai, M. (2005). Reducing agents and imidazole protect Hsv-1 vector from inactivation during immobilized cobalt affinity chromatography. AICHE 2005 annual meeting.Cincinnati, OH.

Jiang, C.P., Wechuck, J.B., Krisky, D., Glorioso, J.C., & Ataai, M. (2005). Immobilized cobalt affinity purification for HSV-1 gene therapy vector. ACS Natl Mtg.San Diego, CA.

Pan, Z.W., Zhu, T., Domagalski, N.R., Koepsel, R., Domach, M., & Ataai, M.M. (2005). Regulating expression of PYK in B subtilis to reduce formation of acidic by-products and improve production of folic acid and recombinant protein. ACS Natl Mtg.San Diego, CA.

Ye, K.M., Jin, S., Ataai, M., & Schultz, J. (2005). One-step purification of retrovirus vectors by IMAC. ACS Natl Mtg.San Diego, CA.

Ataai, M., Jiang, C.P., Krisky, D.M., Goins, W.F., & Glorioso, J.C. (2004). Developing vero cell line expressing cobalt affinity peptide tagged HSV-1 GC for delivering the tag on the virus surface facilitating the virus purification. ACS Natl Mtg.Anaheim, CA.

Zhu, T., Koepsel, R., Domach, M.M., & Ataai, M. (2003). Metabolic engineering of folic acid production. ACS National Meeting.Boston, MA.

Zhu, T., Koepsel, R., Domach, M.M., & Ataai, M.M. (2003). Metabolic engineering of folic acid production. ACS National Meeting.New Orleans.

Ozuer, A., Wechuck, J.B., Goins, W.F., Wolfe, D., Glorioso, J.C., & Ataai, M.M. (2002). Effect of genetic background and culture conditions on the production of herpesvirus-based gene therapy vectors. European Society of Gene Therapy.United States. doi: 10.1002/bit.10162.

Ozuer, A., Wechuck, J.B., Wolfe, D., Goins, F., Wendell, S., Glorioso, J.C., & Ataai, M.M. (2002). Production of replication-defective HSV-1 viral vectors and development of rapid, and precise assay systems to analyze defective and infective particles. ACS National Meeting.Boston, MA.

Ozuer, A., Wechuck, J.B., Wolfe, D., Goins, W.F., Wendell, S., Glorioso, J.C., & Ataai, M.M. (2002). Efficient production and analysis of clinical-grade HSV-1 based gene therapy vectors. ACS National Meeting.Boston, MA.

Lee, S., Phalakornkule, C., Domach, M.M., & Grossmann, I.E. (2000). Recursive MILP model for finding all the alternate optima in LP models for metabolic networks. ACS Natl Mtg.San Francisco, CA. doi: 10.1016/S0098-1354(00)00323-9.

Chen, Y., Pasquinelli, R.S., Ataai, M.M., Koepsel, R.R., & Sheperd, R.E. (1998). Study of the coordination of the high affinity six-mer peptide Ser-Pro-His-His-Gly-Gly to a [Pd-II(methyliminodiacetate)] model of the Cu-II(iminodiacetate) chromatographic binding site. ACS Natl. Mtg..Boston.

Wechuck, J.B., Oligino, T., Glorioso, J.C., & Ataai, M.M. (1998). Efficient strategies for high titer production of viral vectors: Hsv-1/vero cells as a case study. ACS Natl. Mtg..Boston.

Ataai, M.M., Holder, G.D., & Toplak, R.F. (1997). Research experience for undergraduates at the University of Pittsburgh department of chemical and petroleum engineering. In Proceedings - Frontiers in Education Conference, 3, (pp. 1140-1145).

Beitle, R.R., & Ataai, M.M. (1992). Immobilized metal affinity chromatography and related techniques. In AIChE Symposium Series, 88(290), (pp. 34-44).

Ataai, M.M. (1986). MATHEMATICAL MODEL FOR REPLICATION OF R1 PLASMIDS. In American Institute of Chemical Engineers, National Meeting.

Ataai, M.M., & Shuler, M.L. (1985). MATHEMATICAL MODEL FOR PREDICTION OF COPY NUMBER AND PROTEIN PRODUCTION IN E. COLI. In Annual Meeting - American Institute of Chemical Engineers.

Shuler, M.L., Ataai, M.M., & Good, T.A. (1984). COMPUTER MODELS OF INDIVIDUAL BACTERIAL CELLS: A POSSIBLE TOOL FOR TESTING BIOCHEMICAL HYPOTHESES. In Annual Meeting - American Institute of Chemical Engineers.

Research interests

Cell Culture
Metabolism and Fermentation
Protein Purification