Andrew Duncan

  • Wilson, S.R., Delgado, E.R., Alencastro, F., Loewenstein, R.L., Leek, M.P., Peters, L.R., Kamel, K., Wilkinson, P.D., Jain, S., Locker, J., Liu, S., Bhushan, B., & Duncan, A.W. (2026). Diploid hepatocytes resist acetaminophen-induced liver injury through suppressed JNK signaling. Cell Death Dis, 17(1), 203.Springer Nature. doi: 10.1038/s41419-026-08448-z.
  • Asokakumar, A., Mathur, B., Chau, A., Cronologia, B., Alencastro, F., Wheeler, L., Mathews, C.K., Moore, D.D., Duncan, A.W., & Anakk, S. (2025). Constitutive Androstane Receptor induces Ribonucleotide Reductase-M2 expression and maintains hepatocyte ploidy in mice. In bioRxiv. doi: 10.1101/2025.04.29.651109.
  • Bangru, S., Chen, J., Baker, N., Das, D., Chembazhi, U.V., Derham, J.M., Chorghade, S., Arif, W., Alencastro, F., Duncan, A.W., Carstens, R.P., & Kalsotra, A. (2025). ESRP2-microRNA-122 axis promotes the postnatal onset of liver polyploidization and maturation. Genes Dev, 39(5-6), 325-347.Cold Spring Harbor Laboratory. doi: 10.1101/gad.352129.124.
  • Duncan, A.W. (2025). Pathological polyploidy and liver repair failure in RAD51-deficient mice. Hepatology, 81(2), 393-395.Wolters Kluwer. doi: 10.1097/HEP.0000000000000871.
  • Wilson, S.R., Delgado, E.R., Alencastro, F., Loewenstein, R.L., Leek, M.P., Peters, L.R., Kamel, K., Wilkinson, P.D., Jain, S., Locker, J., Liu, S., Bhushan, B., & Duncan, A.W. (2025). Diploid Hepatocytes Resist Acetaminophen-Induced Liver Injury Through Suppressed JNK Signaling. bioRxiv, 2025.07.31.667940.Cold Spring Harbor Laboratory. doi: 10.1101/2025.07.31.667940.
  • Wilson, S.R., & Duncan, A.W. (2023). The Ploidy State as a Determinant of Hepatocyte Proliferation. Semin Liver Dis, 43(4), 460-471.Thieme. doi: 10.1055/a-2211-2144.
  • Xu, L., Paine, A.C., Barbeau, D.J., Alencastro, F., Duncan, A.W., & McElroy, A.K. (2023). Limiting viral replication in hepatocytes alters Rift Valley fever virus disease manifestations. In Gallagher, T. (Ed.). J Virol, 97(9), e0085323.American Society for Microbiology. doi: 10.1128/jvi.00853-23.
  • Ayoob, J.C., Boyce, R.D., Livshits, S., Bruno, T.C., Delgoffe, G.M., Galson, D.L., Duncan, A.W., Atkinson, J.M., Oesterreich, S., Evans, S., Alikhani, M., Baker, T.A., Pratt, S., DeHaan, K.J., Chen, Y., & Boone, D.N. (2022). Getting to YES: The Evolution of the University of Pittsburgh Medical Center Hillman Cancer Center Youth Enjoy Science (YES) Academy. J STEM Outreach, 5(2).Vanderbilt University Library. doi: 10.15695/jstem/v5i2.02.
  • Barajas, J.M., Lin, C.H., Sun, H.L., Alencastro, F., Zhu, A.C., Aljuhani, M., Navari, L., Yilmaz, S.A., Yu, L., Corps, K., He, C., Duncan, A.W., & Ghoshal, K. (2022). METTL3 Regulates Liver Homeostasis, Hepatocyte Ploidy, and Circadian Rhythm-Controlled Gene Expression in Mice. Am J Pathol, 192(1), 56-71.Elsevier. doi: 10.1016/j.ajpath.2021.09.005.
  • Ma, J., Tan, X., Kwon, Y., Delgado, E.R., Zarnegar, A., DeFrances, M.C., Duncan, A.W., & Zarnegar, R. (2022). A Novel Humanized Model of NASH and Its Treatment With META4, A Potent Agonist of MET. Cell Mol Gastroenterol Hepatol, 13(2), 565-582.Elsevier. doi: 10.1016/j.jcmgh.2021.10.007.
  • Roy, N., Alencastro, F., Roseman, B.A., Wilson, S.R., Delgado, E.R., May, M.C., Bhushan, B., Bello, F.M., Jurczak, M.J., Shiva, S., Locker, J., Gingras, S., & Duncan, A.W. (2022). Dysregulation of Lipid and Glucose Homeostasis in Hepatocyte-Specific SLC25A34 Knockout Mice. Am J Pathol, 192(9), 1259-1281.Elsevier. doi: 10.1016/j.ajpath.2022.06.002.
  • Wang, H., Lu, J., Alencastro, F., Roberts, A., Fiedor, J., Carroll, P., Eisenman, R.N., Ranganathan, S., Torbenson, M., Duncan, A.W., & Prochownik, E.V. (2022). Coordinated Cross-Talk Between the Myc and Mlx Networks in Liver Regeneration and Neoplasia. Cell Mol Gastroenterol Hepatol, 13(6), 1785-1804.Elsevier. doi: 10.1016/j.jcmgh.2022.02.018.
  • Wilson, S.R., & Duncan, A.W. (2022). Single-Cell DNA Sequencing Reveals Chromosomal Diversity in HCC and a Novel Model of HCC Evolution. Gastroenterology, 162(1), 46-48.Elsevier. doi: 10.1053/j.gastro.2021.09.065.
  • Delgado, E.R., Erickson, H.L., Tao, J., Monga, S.P., Duncan, A.W., & Anakk, S. (2021). Scaffolding Protein IQGAP1 Is Dispensable, but Its Overexpression Promotes Hepatocellular Carcinoma via YAP1 Signaling. Mol Cell Biol, 41(4).Taylor & Francis. doi: 10.1128/MCB.00596-20.
  • Wang, H., Lu, J., Alencastro, F., Roberts, A., Fiedor, J., Carroll, P., Eisenman, R.N., Ranganathan, S., Torbenson, M., Duncan, A.W., & Prochownik, E.V. (2021). Coordinated Cross-Talk Between the Myc and Mlx Networks in Liver Regeneration and Neoplasia. 2021.08.05.455215.Cold Spring Harbor Laboratory. doi: 10.1101/2021.08.05.455215.
  • Wilkinson, P.D., & Duncan, A.W. (2021). Differential Roles for Diploid and Polyploid Hepatocytes in Acute and Chronic Liver Injury. Semin Liver Dis, 41(1), 42-49.Thieme. doi: 10.1055/s-0040-1719175.
  • Duncan, A.W. (2020). Single-Cell and Bulk Transcriptome Profiling Reveals Unique Features of Diploid and Polyploid Hepatocytes. Cell Mol Gastroenterol Hepatol, 9(1), 193-194.Elsevier. doi: 10.1016/j.jcmgh.2019.09.008.
  • Duncan, A.W. (2020). Hepatocyte ploidy modulation in liver cancer. EMBO Rep, 21(12), e51922.Springer Nature. doi: 10.15252/embr.202051922.
  • Gliozzi, M.L., Espiritu, E.B., Shipman, K.E., Rbaibi, Y., Long, K.R., Roy, N., Duncan, A.W., Lazzara, M.J., Hukriede, N.A., Baty, C.J., & Weisz, O.A. (2020). Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model. J Am Soc Nephrol, 31(1), 67-83.Wolters Kluwer. doi: 10.1681/ASN.2019020125.
  • Stahl, E.C., Delgado, E.R., Alencastro, F., LoPresti, S.T., Wilkinson, P.D., Roy, N., Haschak, M.J., Skillen, C.D., Monga, S.P., Duncan, A.W., & Brown, B.N. (2020). Inflammation and Ectopic Fat Deposition in the Aging Murine Liver Is Influenced by CCR2. Am J Pathol, 190(2), 372-387.Elsevier. doi: 10.1016/j.ajpath.2019.10.016.
  • Takeishi, K., Collin de l'Hortet, A., Wang, Y., Handa, K., Guzman-Lepe, J., Matsubara, K., Morita, K., Jang, S., Haep, N., Florentino, R.M., Yuan, F., Fukumitsu, K., Tobita, K., Sun, W., Franks, J., Delgado, E.R., Shapiro, E.M., Fraunhoffer, N.A., Duncan, A.W., Yagi, H., Mashimo, T., Fox, I.J., & Soto-Gutierrez, A. (2020). Assembly and Function of a Bioengineered Human Liver for Transplantation Generated Solely from Induced Pluripotent Stem Cells. Cell Rep, 31(9), 107711.Elsevier. doi: 10.1016/j.celrep.2020.107711.
  • Xue, Y., Bhushan, B., Mars, W.M., Bowen, W., Tao, J., Orr, A., Stoops, J., Yu, Y., Luo, J., Duncan, A.W., & Michalopoulos, G.K. (2020). Phosphorylated Ezrin (Thr567) Regulates Hippo Pathway and Yes-Associated Protein (Yap) in Liver. Am J Pathol, 190(7), 1427-1437.Elsevier. doi: 10.1016/j.ajpath.2020.03.014.
  • Wilkinson, P.D., Alencastro, F., Delgado, E.R., Leek, M.P., Weirich, M.P., Otero, P.A., Roy, N., Brown, W.K., Oertel, M., & Duncan, A.W. (2019). Polyploid Hepatocytes Facilitate Adaptation and Regeneration to Chronic Liver Injury. Am J Pathol, 189(6), 1241-1255.Elsevier. doi: 10.1016/j.ajpath.2019.02.008.
  • Wilkinson, P.D., Delgado, E.R., Alencastro, F., Leek, M.P., Roy, N., Weirich, M.P., Stahl, E.C., Otero, P.A., Chen, M.I., Brown, W.K., & Duncan, A.W. (2019). The Polyploid State Restricts Hepatocyte Proliferation and Liver Regeneration in Mice. Hepatology, 69(3), 1242-1258.Wolters Kluwer. doi: 10.1002/hep.30286.
  • Wang, H., Dolezal, J.M., Kulkarni, S., Lu, J., Mandel, J., Jackson, L.E., Alencastro, F., Duncan, A.W., & Prochownik, E.V. (2018). Myc and ChREBP transcription factors cooperatively regulate normal and neoplastic hepatocyte proliferation in mice. J Biol Chem, 293(38), 14740-14757.Elsevier. doi: 10.1074/jbc.RA118.004099.
  • Jackson, L.E., Kulkarni, S., Wang, H., Lu, J., Dolezal, J.M., Bharathi, S.S., Ranganathan, S., Patel, M.S., Deshpande, R., Alencastro, F., Wendell, S.G., Goetzman, E.S., Duncan, A.W., & Prochownik, E.V. (2017). Genetic Dissociation of Glycolysis and the TCA Cycle Affects Neither Normal nor Neoplastic Proliferation. Cancer Res, 77(21), 5795-5807.American Association for Cancer Research (AACR). doi: 10.1158/0008-5472.CAN-17-1325.
  • Edmunds, L.R., Otero, P.A., Sharma, L., D'Souza, S., Dolezal, J.M., David, S., Lu, J., Lamm, L., Basantani, M., Zhang, P., Sipula, I.J., Li, L., Zeng, X., Ding, Y., Ding, F., Beck, M.E., Vockley, J., Monga, S.P.S., Kershaw, E.E., O'Doherty, R.M., Kratz, L.E., Yates, N.A., Goetzman, E.P., Scott, D., Duncan, A.W., & Prochownik, E.V. (2016). Abnormal lipid processing but normal long-term repopulation potential of myc-/- hepatocytes. Oncotarget, 7(21), 30379-30395.Impact Journals. doi: 10.18632/oncotarget.8856.
  • Hsu, S.H., Delgado, E.R., Otero, P.A., Teng, K.Y., Kutay, H., Meehan, K.M., Moroney, J.B., Monga, J.K., Hand, N.J., Friedman, J.R., Ghoshal, K., & Duncan, A.W. (2016). MicroRNA-122 regulates polyploidization in the murine liver. Hepatology, 64(2), 599-615.Wolters Kluwer. doi: 10.1002/hep.28573.
  • Duncan, A.W. (2015). Changes in Hepatocyte Ploidy During Liver Regeneration. In Liver Regeneration. (pp. 167-184).Elsevier. doi: 10.1016/b978-0-12-420128-6.00012-9.
  • Hsu, S.H., & Duncan, A.W. (2015). Pathological polyploidy in liver disease. Hepatology, 62(3), 968-970.Wolters Kluwer. doi: 10.1002/hep.27908.
  • Duncan, A.W. (2013). Aneuploidy, polyploidy and ploidy reversal in the liver. Semin Cell Dev Biol, 24(4), 347-356.Elsevier. doi: 10.1016/j.semcdb.2013.01.003.
  • Duncan, A.W., & Soto-Gutierrez, A. (2013). Liver repopulation and regeneration: new approaches to old questions. Curr Opin Organ Transplant, 18(2), 197-202.Wolters Kluwer. doi: 10.1097/MOT.0b013e32835f07e2.
  • Kurinna, S., Stratton, S.A., Coban, Z., Schumacher, J.M., Grompe, M., Duncan, A.W., & Barton, M.C. (2013). p53 regulates a mitotic transcription program and determines ploidy in normal mouse liver. Hepatology, 57(5), 2004-2013.Wolters Kluwer. doi: 10.1002/hep.26233.
  • Duncan, A.W., Hanlon Newell, A.E., Bi, W., Finegold, M.J., Olson, S.B., Beaudet, A.L., & Grompe, M. (2012). Aneuploidy as a mechanism for stress-induced liver adaptation. J Clin Invest, 122(9), 3307-3315.American Society for Clinical Investigation. doi: 10.1172/JCI64026.
  • Duncan, A.W., Hanlon Newell, A.E., Smith, L., Wilson, E.M., Olson, S.B., Thayer, M.J., Strom, S.C., & Grompe, M. (2012). Frequent aneuploidy among normal human hepatocytes. Gastroenterology, 142(1), 25-28.Elsevier. doi: 10.1053/j.gastro.2011.10.029.
  • Dorrell, C., Erker, L., Schug, J., Kopp, J.L., Canaday, P.S., Fox, A.J., Smirnova, O., Duncan, A.W., Finegold, M.J., Sander, M., Kaestner, K.H., & Grompe, M. (2011). Prospective isolation of a bipotential clonogenic liver progenitor cell in adult mice. Genes Dev, 25(11), 1193-1203.Cold Spring Harbor Laboratory. doi: 10.1101/gad.2029411.
  • Duncan, A.W., Taylor, M.H., Hickey, R.D., Hanlon Newell, A.E., Lenzi, M.L., Olson, S.B., Finegold, M.J., & Grompe, M. (2010). The ploidy conveyor of mature hepatocytes as a source of genetic variation. Nature, 467(7316), 707-710.Springer Nature. doi: 10.1038/nature09414.
  • Zhang, Q.S., Marquez-Loza, L., Eaton, L., Duncan, A.W., Goldman, D.C., Anur, P., Watanabe-Smith, K., Rathbun, R.K., Fleming, W.H., Bagby, G.C., & Grompe, M. (2010). Fancd2-/- mice have hematopoietic defects that can be partially corrected by resveratrol. Blood, 116(24), 5140-5148.American Society of Hematology. doi: 10.1182/blood-2010-04-278226.
  • Duncan, A.W., Dorrell, C., & Grompe, M. (2009). Stem cells and liver regeneration. Gastroenterology, 137(2), 466-481.Elsevier. doi: 10.1053/j.gastro.2009.05.044.
  • Duncan, A.W., Hickey, R.D., Paulk, N.K., Culberson, A.J., Olson, S.B., Finegold, M.J., & Grompe, M. (2009). Ploidy reductions in murine fusion-derived hepatocytes. In Cohen-Fix, O. (Ed.). PLoS Genet, 5(2), e1000385.Public Library of Science (PLoS). doi: 10.1371/journal.pgen.1000385.
  • DiMascio, L., Voermans, C., Uqoezwa, M., Duncan, A., Lu, D., Wu, J., Sankar, U., & Reya, T. (2007). Identification of adiponectin as a novel hemopoietic stem cell growth factor. J Immunol, 178(6), 3511-3520.Oxford University Press (OUP). doi: 10.4049/jimmunol.178.6.3511.
  • Duncan, A.W., Rattis, F.M., DiMascio, L.N., Congdon, K.L., Pazianos, G., Zhao, C., Yoon, K., Cook, J.M., Willert, K., Gaiano, N., & Reya, T. (2005). Integration of Notch and Wnt signaling in hematopoietic stem cell maintenance. Nat Immunol, 6(3), 314-322.Springer Nature. doi: 10.1038/ni1164.
  • Kitsos, C.M., Sankar, U., Illario, M., Colomer-Font, J.M., Duncan, A.W., Ribar, T.J., Reya, T., & Means, A.R. (2005). Calmodulin-dependent protein kinase IV regulates hematopoietic stem cell maintenance. J Biol Chem, 280(39), 33101-33108.Elsevier. doi: 10.1074/jbc.M505208200.
  • Reya, T., Duncan, A.W., Ailles, L., Domen, J., Scherer, D.C., Willert, K., Hintz, L., Nusse, R., & Weissman, I.L. (2003). A role for Wnt signalling in self-renewal of haematopoietic stem cells. Nature, 423(6938), 409-414.Springer Nature. doi: 10.1038/nature01593.
  • Willert, K., Brown, J.D., Danenberg, E., Duncan, A.W., Weissman, I.L., Reya, T., Yates, J.R., & Nusse, R. (2003). Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature, 423(6938), 448-452.Springer Nature. doi: 10.1038/nature01611.
  • Cao, Y., Janssen, E.M., Duncan, A.W., Altman, A., Billadeau, D.D., & Abraham, R.T. (2002). Pleiotropic defects in TCR signaling in a Vav-1-null Jurkat T-cell line. EMBO J, 21(18), 4809-4819.Springer Nature. doi: 10.1093/emboj/cdf499.
  • BaSalamah, M.A., Marr, H.S., Duncan, A.W., & Edgell, C.J. (2001). Testican in human blood. Biochem Biophys Res Commun, 283(5), 1083-1090.Elsevier. doi: 10.1006/bbrc.2001.4900.
  • Marr, H.S., Basalamah, M.A., Bouldin, T.W., Duncan, A.W., & Edgell, C.J. (2000). Distribution of testican expression in human brain. Cell Tissue Res, 302(2), 139-144.Springer Nature. doi: 10.1007/s004410000277.

  • Roy, N., May, M., Delgado, E., Alencastro, F., Wilkinson, P., Smyers, M., Reynolds, M., Shiva, S., & Duncan, A. (2020). SLC25A34 regulates bioenergetic metabolism in the murine liver. In FASEB JOURNAL, 34(S1).Wiley. doi: 10.1096/fasebj.2020.34.s1.03444.
  • Erickson, H., Delgado, E., Duncan, A.W., & Anakk, S. (2019). DELETION AND OVER EXPRESSION OF SCAFFOLD PROTEIN IQGAP1 PROMOTES HEPATOCELLULAR CARCINOMA. In HEPATOLOGY, 70, (pp. 1202A-1203A).
  • Delgado, E.R., & Duncan, A.W. (2017). Regulation of Oncogenic Signaling Pathways in Hepatocellular Carcinoma by the Pleiotropic Scaffold Protein IQGAP1. In AMERICAN JOURNAL OF PATHOLOGY, 187(10), (pp. 2369-2370).
  • Khan, Z., Zhang, P., Bowen, W.C., Alencastro, F., Duncan, A.W., Stolz, D.B., & Michalopoulos, G.K. (2017). Age-dependent effects of intracellular ATZ protein globules on hepatocyte proliferation and ploidy in alpha-1 antitrypsin deficiency. In HEPATOLOGY, 66, (p. 716A).
  • Roy, N., Wilkinson, P.D., Delgado, E.R., Alencastro, F., Leek, M.P., Corey, C.G., Shiva, S., & Duncan, A.W. (2017). A Role for SLC25A34, a Putative Oxaloacetate Carrier, in Non-Alcoholic Fatty Liver Disease. In AMERICAN JOURNAL OF PATHOLOGY, 187(10), (pp. 2368-2369).
  • Wilkinson, P.D., Otero, P., Alencastro, F., Delgado, E.R., Roy, N., Leek, M., Weirich, M.P., Oertel, M., & Duncan, A.W. (2017). A Role for Polyploid Hepatocytes in Liver Repopulation and Adaptation to Chronic Injury. In AMERICAN JOURNAL OF PATHOLOGY, 187(10), (p. 2366).
  • Hsu, S.H., Delgado, E., Otero, P.A., Meehan, K., Moroney, J.B., Teng, K.Y., Ghoshal, K., & Duncan, A.W. (2015). MicroRNA-122 (miR-122) Regulates Polyploidy in the Liver. In HEPATOLOGY, 62, (p. 457A).
Research Interests
Biochemistry and cell biology Clinical sciences Immunology Medical biotechnology Genetics Cardiovascular medicine and haematology
headshot of Andrew Duncan
Contact Andrew Duncan
Associate Professor
duncana@pitt.edu
Work: (412) 624-5302