Guang Li

Heart development as a critical embryonic developmental process is tightly regulated on the cellular and molecular level. If this process goes awry, it will lead to congenital heart diseases (CHD), which accounts for a significant portion of stillbirths and is present in 1-2% of all live births. The Li lab is using advanced techniques including human induced pluripotent stem cells (hiPSC), single-cell RNA sequencing, single molecular in situ hybridization, CRISPR/Cas9, and tissue cleaning methods to decode the spatial and temporal information of each single cardiac cells. Meanwhile, with the knowledge gained from the study of basic cardiac lineage regulations, the lab is also exploring the lineage defects in congenital heart diseases using patient-derived iPSCs.

  • PhD, Chinese Academy of Sciences, 2007 - 2012

  • Feng, W., Bais, A., He, H., Rios, C., Jiang, S., Xu, J., Chang, C., Kostka, D., & Li, G. (2022). Single-cell transcriptomic analysis identifies murine heart molecular features at embryonic and neonatal stages. Nat Commun, 13(1), 7960.Springer Nature. doi: 10.1038/s41467-022-35691-7.
  • Feng, W., Schriever, H., Jiang, S., Bais, A., Wu, H., Kostka, D., & Li, G. (2022). Computational profiling of hiPSC-derived heart organoids reveals chamber defects associated with NKX2-5 deficiency. Commun Biol, 5(1), 399.Springer Nature. doi: 10.1038/s42003-022-03346-4.
  • Feng, W., Przysinda, A., & Li, G. (2020). Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells. J Vis Exp, 2020(155).MyJove. doi: 10.3791/60647.
  • Przysinda, A., Feng, W., & Li, G. (2020). Diversity of Organism-Wide and Organ-Specific Endothelial Cells. Curr Cardiol Rep, 22(4), 19.Springer Nature. doi: 10.1007/s11886-020-1275-9.
  • Li, G., Tian, L., Goodyer, W., Kort, E.J., Buikema, J.W., Xu, A., Wu, J.C., Jovinge, S., & Wu, S.M. (2019). Single cell expression analysis reveals anatomical and cell cycle-dependent transcriptional shifts during heart development. In Klein, A., & Treutlein, B. (Eds.). Development, 146(12).The Company of Biologists. doi: 10.1242/dev.173476.
Research Interests
Cardiovascular medicine and haematology Genetics
headshot of Guang Li
Contact Guang Li
Associate Professor
GUANGLI@pitt.edu
Bioengineering Department