Pitt | Swanson Engineering
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Feb

Feb
4
2019

Pitt Power Engineering Seniors Nathan Carnovale and Shamus O’Haire named IEEE PES Scholars

Electrical & Computer

PITTSBURGH (February 4, 2019) … The Institute of Electrical and Electronics Engineers (IEEE) Power and Energy Society (PES) selected University of Pittsburgh seniors Nathan Carnovale and Shamus (James) O’Haire as recipients of the 2018-19 IEEE PES Scholarship Plus Award. Both are majoring in electrical and computer engineering at Pitt’s Swanson School of Engineering. This is Mr. Carnovale’s second IEEE PES Scholarship in as many years. “Being named an IEEE PES Scholar is well-respected in the field of power engineering, and both Nate and Shamus are outstanding ambassadors for our program,” said Robert Kerestes, assistant professor of electrical and computer engineering at Pitt. “We are incredibly proud of their accomplishments and I think they have great potential in their future careers.”The IEEE PES Scholarship Plus Initiative awarded scholarships to 174 electrical engineering students from 96 universities across the U.S., Canada, and Puerto Rico. Applicants for the scholarships were evaluated based on high achievement with a strong GPA, distinctive extracurricular commitments, and dedication to the power and energy field. Over the past seven years, the Scholarship Plus Initiative has awarded more than $3.5 million in scholarships to students interested in pursuing a career in the power and energy industry. Carnovale and O’Haire are the Swanson School’s 11th and 12th PES recipients since the scholarship’s inception in 2011 and continue the School’s seven-year streak of at least one awardee each year. Also, according to IEEE, Pitt is one of only 16 universities that have had at least one recipient every year since 2011. About Nathan CarnovaleNate Carnovale is scheduled to graduate from the University of Pittsburgh in December 2019 with a bachelor of science in electrical engineering and a concentration in electric power, and plans to pursue an M.S. degree in electric power engineering at Pitt starting in spring 2020. During his undergraduate career, he interned with Eaton for two summers, working at Eaton’s Power Systems Experience Center and in Eaton’s Power Systems Automation services group in Warrendale, PA. There he gained experience in power systems metering and monitoring, as well as experience installing, wiring, and programming Eaton demos at the Experience Center. He will be working in Eaton’s Power Systems Controls group this summer working with microgrids. For four semesters at Pitt, Carnovale has been a teaching assistant for the Art of Making, an introductory engineering course to hands-on systems design. He is currently working to develop an adapted physical education learning tool for students with physical and mental challenges at the Western Pennsylvania School for Blind Children in Pittsburgh, a project he started during his time as a student in the Art of Making course.About Shamus (James) O’HaireShamus O’Haire is scheduled to graduate in spring 2019 with a bachelor of science degree in electrical engineering with a concentration in power systems and a minor in computer science. During his career at Pitt, he has spent three summers interning at Exelon Corp., a Fortune 100 energy company that operates electric generation nationwide as well as electric distribution in the Northeastern US. He gained industry experience in system operations, transmission planning, and substations engineering during his time with the company, and hopes these experiences will be a springboard for his future career in the power and energy industry. O’Haire currently serves as the Chief Electronics Engineer for Pitt Aero Society of Automotive Engineers, and is a member of IEEE. ###

Jan

Jan
29
2019

Lights, Camera, Action: Pitt iGEM team captures silver medal for their “Molecular Movie Camera”

Bioengineering, Electrical & Computer, Student Profiles

PITTSBURGH (January 29, 2019) … The ability to measure and record molecular signals in a cell can help researchers better understand its behavior, but current systems are limited and provide only a “snapshot” of the environment rather than a more informative timeline of cellular events. In an effort to give researchers a complete understanding of event order, a team of University of Pittsburgh undergraduate students prototyped a frame-by-frame “video” recording device using bacteria. The group created this project for the 2018 International Genetically Engineered Machine (iGEM) competition, an annual synthetic biology research competition in which over 300 teams from around the world design and carry out projects to solve an open research or societal problem. The Pitt undergraduate group received a silver medal for their device titled “CUTSCENE.” The iGEM team included two Swanson School of Engineering students: Evan Becker, a junior electrical engineering student, and Vivian Hu, a junior bioengineering student. Other team members included Matthew Greenwald, a senior microbiology student; Tucker Pavelek, a junior molecular biology and physics student; Libby Pinto, a sophomore microbiology and political science student; and Zemeng Wei, a senior chemistry student. CUTSCENE aims to show a “video” of cellular activity by recording events in the cell using modified CRISPR/Cas9 technology. Hu said, “By knowing what time molecular events are happening inside of a cell, we are able to better understand a cell's history and how it responds to external stimuli.” Their system improved upon older methods that could only record the levels of stimuli at a single point in time. They used a movie analogy to illustrate their objective. “Try guessing the plot of a movie by looking at the poster; you can get an idea of what is going on, but to really understand the story, you need to watch the film,” said Becker. “Unless researchers are taking many snapshots of the cellular activity over time, the image doesn’t give any sense of causality. You can see that the molecule is there, but you don't know where it has been or where it is going.” For their project, the iGEM team used modified CRISPR/Cas9 technology called a base editor. The CRISPR/Cas9 system contains two key components: a guideRNA (gRNA) that matches a specific sequence of DNA and a Cas9 protein that makes a cut at the specific sequence, ultimately leading to the insertion or deletion of base pairs - the building blocks of DNA. In addition to these components, a CRISPR/Cas9 base editor contains an enzyme called cytidine deaminase that is able to make a known single nucleotide mutation at a desired location of DNA. “We achieved a method of true chronological event recording by introducing recording plasmids with repeating units of DNA and multiple gRNA to direct our base editor construct,” said Hu. “This technique will provide an understanding of the order in which molecules and proteins appear in systems.” “A recording plasmid can be thought of as a roll of unexposed film, with each frame being an identical sequence of DNA,” explained Wei. “A single-guideRNA (sgRNA) directs the CRISPR/Cas9 base editor to move along the recording plasmid, making mutations at a timed rate and constantly shifting which frame is in front of our base editor. Activated by the presence of a stimulus, another sgRNA can mark the current frame.” The iGEM team’s approach to this technology will allow them to figure out which molecules are abundant at specific times and perhaps reveal hidden, causal relationships. The information gathered from the device has many potential applications and may allow researchers to develop medicines and therapies based on the timing of the cellular malfunction. “The team did a tremendous amount of lab work over the summer, implementing the cellular event recording methodology,” said Alex Deiters, a professor of chemistry at Pitt who helped advise the iGEM team. “Most importantly, the students developed this clever idea on their own by first identifying a current technology gap and then applying modern gene editing machinery to it. The silver medal is well-deserved!” In addition to Dr. Deiters, the 2018 Pitt iGEM team was advised by Dr. Jason Lohmueller, American Cancer Society Postdoctoral Fellow in the Department of Immunology; Dr. Natasa Miskov-Zivanov, Assistant Professor of Electrical and Computer Engineering, Bioengineering, and Computational and Systems Biology; Dr. Sanjeev Shroff, Distinguished Professor and Gerald E. McGinnis Chair of Bioengineering; and Dr. Cheryl Telmer, a Research Biologist at Carnegie Mellon University. Funding for the 2018 Pitt iGEM effort was provided by the University of Pittsburgh (Office of the Senior Vice Chancellor for Research, Honors College, Kenneth P. Dietrich School of Arts and Sciences, Department of Biological Sciences, Department of Chemistry, Swanson School of Engineering, Department of Bioengineering, and Department of Electrical & Computer Engineering), New England Biolabs (NEB), and Integrated DNA Technologies (IDT). ###