Anne Robertson, PhD
The Bio Tissues and Complex Fluids Laboratory, directed by Anne Robertson, PhD, is devoted to the characterization and experimental study of complex materials. Much of the work in this laboratory focuses on understanding and quantifying the link between material behavior and structure in soft biological tissues such as the walls of arteries- in both health and disease. We use experimental, theoretical and computational tools for these investigations. For example, we have used multiphoton microscopy coupled with mechanical testing to image the gradual recruitment of collagen fibers in the artery wall during mechanical loading. This coupled approach enabled us to clarify the role of collagen fibers in determining the nonlinear mechanical properties of the artery wall. We then used this data to develop structurally motivated constitutive models of the artery wall that can be used in computational studies of the damage caused during medical treatments such as balloon angioplasty.
Jack Patzer, PhDpatzer@pitt.edu
This laboratory is under the direction of Jack Patzer, PhD and focuses on research related to the application of BioThemodynamics and BioTransport Phenomena (principles of heat, momentum, and mass transport) to understanding the properties of physiological systems, medical devices, and bioreactor engineering. Current investigations involve the application bound solute dialysis (BSD) as a detoxification approach to support patients with liver failure, use of ischemia protective polymers (IPP) to mitigate ischemia/reperfusion injury in organ harvest and transplant, and wound perfusion/skin regeneration for patients with severe burns. Major equipment includes a Sun workstation for finite element analysis of fluid dynamics, spectrophotometers for colorimetric composition analysis, plate reader for colorimetric composition analysis, blood-gas analyzer, table-top refrigerated centrifuge, cell incubators, and Prisma dialysis machines. Other equipment includes multiple roller pumps, gas mass flow controllers, oscilloscope, electrochemistry controllers and analyzers.
This lab is directed by Dr. Youngjae Chun and its objective is to design, manufacture, and test medical devices for treating vascular diseases. Primary research focuses on improving device performance and developing more diverse biomedical applications for treating vascular diseases with a focus on novel materials and manufacturing concepts. This lab also focuses on developing novel artificial biomaterials such as fully biocompatible hybrid/composite materials made of metals, polymers, and bio-species. Facilities include
pulsatile flow circuits with vascular disease models, cell-tissue culture capabilities, and florescent microscopy with imaging system. Current research is focused on the development of (1) a novel biomaterials and biocompatible surface modification processes, (2) minimally invasive surgical solutions that include smart stent, stent graft, and guidewire, and (3) low-profile mechanical prosthetics.
Yadong Wang, PhD
This laboratory, under the direction of Yadong Wang, PhD, works at the interface of chemistry, materials, and medicine. The research focus is on creating biomaterials that present controlled chemical, physical, and mechanical signals to the biological systems. The ultimate goal is to direct how human bodies will interact with these materials in a therapeutic environment. The laboratory actively engages in collaborative efforts to explore the applications of these materials in cardiovascular tissue engineering, nerve regeneration, and controlled release of therapeutics. The major equipment of the laboratory includes essential tools for chemical synthesis (inert atmosphere box, GPC, microwave synthesis station) and cell biology (plate reader, microscope, RT PCR).
Current Frank cluster hardware
Bryan Brown, PhDbrownb@upmc.edu
The Brown Laboratory is a newly established space housed within the BSP2 of the McGowan Institute for Regenerative Medicine. The focus of the laboratory is on the role of the host immune response to implantable biomaterials. The phenotype and function of host innate immune cells is of particular interest, and has been shown to be a predictor of the success of biomaterials based strategies for tissue reconstruction. The Brown Laboratory also participates in new biomaterials development and identification of biomaterials for clinical applications. The Brown Laboratory is equipped for both in vitro cell culture and assessment of samples from in vivo experimentation.
This laboratory is under the direction of Sanjeev Shroff, PhD and focuses on research related to cardiovascular mechano-energetics and structure-function relationships. This research utilizes a variety of biophysical, cell and molecular biology, biochemistry, and imaging techniques. The facility has: 1) setups for biophysical measurements at isolated heart, isolated muscle, and single cell levels (mechanics and intracellular calcium transients), 2) a cell-culture room (incubator, laminar flow hood, centrifuge, microscope), and 3) a wet lab which has equipment necessary to do protein biochemistry and molecular biology research.
Partha Roy, PhD
This research laboratory is under the direction of Partha Roy, PhD, and offers graduate and undergraduate students the ability to participate in research related to molecular mechanisms of cell migration with emphasis in tumor metastasis and angiogenesis. This research utilizes a variety of cell biology, molecular biology, biochemistry, imaging and in vivo techniques. The facility has: 1) a cell culture room that is equipped with tissue culture incubators, laminar flow hood, centrifuge and a microscope, 2) a wet lab which has equipment necessary to do protein biochemistry and molecular biology research, and 3) a microscopy room that houses an IX-71 Olympus research grade inverted microscope and live-cell image acquisition system.