Pitt | Swanson Engineering
Saik Kia Goh

1178A, Benedum Hall

sag93@pitt.edu

Link to CV

                                                

 

Research Focus

My research is focused on investigating the potential use of native-derived whole organ scaffold as a biomaterial candidate to support pancreatic differentiation of embryonic stem cells. Our long-term goal is to design and show proof of concept of a regenerative medicine strategy that reconstructs functional insulin secreting cells for treatment of diabetes. Recently described concept of whole organ engineering provides a possible avenue by which functional endocrine pancreatic tissue can be created. By using the native pancreatic extracellular matrix as a favorable 3-D microenvironmental niche and combining this 3-D scaffold with relevant cell types, there is a potential that a functional endocrine pancreas can be “manufactured” for therapeutic use. While the importance of native microenvironment in organ functionality is well appreciated, the required extent of specificity of this native microenvironment is still an outstanding question.

Specific aims to support our long-term goal are:

  1. Generation and characterization of perfusion-decellularized whole organ pancreas.

  2. Repopulation and differentiation of hESC in 3D whole organ pancreatic microenvironment

  3. Solubilization of whole organ-derived ECM to evaluate matrix specificity and cell-ECM interaction during pancreatic differentiation

 

Education  

2010-Present  Doctoral Program, Department of Bioengineering, University of Pittsburgh
2006                B.S. Genetics, Cellular and Developmental Biology, University of Minnesota

 

Teaching Experience 

2012 Teaching Assistant: Bioengineering class - BIOENG2521, Molecular Cell Biology and Biophysics I at University of Pittsburgh taught by Dr. Partha Roy.
2011 Teaching Assistant: Bioengineering class - BIOENG2520, Molecular Cell Biology and Biophysics II at University of Pittsburgh taught by Dr. Partha Roy.

 

Publications 

  • Goh SK, Bertera S, Olsen P, Candiello JE, Halfter W, Uechi G, Balasubramani M, Johnson SA, Brian BM, Kollar E, Badylak SF, Banerjee I. Perfusion-decellularized pancreas as a natural 3D scaffold for pancreatic tissue and whole organ engineering. Biomaterials 2013 Sep;34(28):6760-72. 
  • Goh SK, Olsen P, Banerjee I. Extracellular matrix Aggregates from Differentiating Embryoid Bodies as a Scaffold to Support ESC Proliferation and Differentiation. PLoS One. 2013 Apr 18;8(4):e61856
  • Simpson DL, Mishra R, Sharma S, Goh SK, Deshmukh S, Kaushal S. A strong regenerative ability of cardiac stem cells derived from neonatal hearts. Circulation. 2012 Sep 11;126(11 Suppl 1):S46-53.
  • Candiello JE, Jaramillo M, Goh SK, Banerjee I. Role of substrates in diabetes therapy: stem cell differentiation and islet transplantation. Crit Rev Biomed Eng. 2011;39(6):535-55
  • Kaushal S, Patel SK, Goh SK, Sood A, Walker BL, Backer CL. A novel combination of bioresorbable polymeric film and expanded polytetrafluoroethylene provides a protective barrier and reduces adhesions. J Thorac Cardiovasc Surg. 2011 Mar;141(3):789-95.
  • Mishra R, Vijayan K, Colletti EJ, Harrington DA, Matthiesen TS, Simpson D, Goh SK, Walker BL, Almeida-Porada G, Wang D, Backer CL, Dudley SC Jr, Wold LE, Kaushal S. Characterization and functionality of cardiac progenitor cells in congenital heart patients. Circulation. 2011 Feb 1;123(4):364-73. Epub 2011 Jan 17.
  • Medeiros RB, Papenfuss K, Aho J, Goh SK, Elayaperumal A, Resnik E, Ni HT. Novel sequential ChIP and simplified basic ChIP protocols for promoter co-occupancy and target gene identification in human embryonic stem cells. BMC Biotechnology 2009 Jun 29;9:59.
  • Ott HC, Matthiesen TS, Goh SK, Black LB, Kren SM, Netoff TI, Taylor DA. Perfusion Decellularized Matrix: Using Nature’s Platform for Engineering Bioartifical Heart. Nature Medicine. 2008 Feb;14(2):213-21.
  • Ott HC, Matthiesen TS, Brechtken J, Grindle S, Goh SK, Nelson W, Taylor DA. (2007). The adult human heart as a source for stem cells: repair strategies with embryonic-like progenitor cells. Nat Clin Pract Cardiovasc Med. 2007 Feb;4 Suppl 1(S1):S27-S39.
      

 

Conference Presentations  

  • Goh SK, Bertera S, Olsen P, Candiello J, Uechi G, Balasubramani M, Johnson S, Sicare B, Badylak SF Banerjee I. Perfusion-decellularized pancreas as a 3D natural scaffold for pancreatic tissue and whole organ engineering. BMES Annual Meeting, Seattle, accepted for Platform Presentation, 2013
  • Goh SK, Bertera S, Olsen P, Candiello J, Uechi G, Balasubramani M, Johnson S, Sicare B, Badylak SF Banerjee I. Perfusion-decellularized matrix as a natural scaffold for human embryonic stem cells differentiation and maturation. BMES Annual Meeting, Seattle, accepted for Poster Presentation, 2013
  • Goh SK, Bertera S, Banerjee I. In-vivo Maturation of Human Embryonic Stem Cells derived Endocrine Pancreas in Perfusion-Decellularized Pancreas. International Society for Stem Cells Research (ISSCR) 11 Annual Meeting, Boston, accepted for Poster Presentation, 2013.
  • Goh SK, Bertera S, Banerjee I. Whole organ 3D microenvironment as a regulatory cue for pancreatic differentiation of embryonic stem cells. BMES Annual Meeting, Atlanta, accepted for Poster Presentation, 2012.
  • Goh SK, Bertera S, Banerjee I. Whole organ 3D microenvironment as a regulatory cue for pancreatic differentiation of embryonic stem cells. AIChE Annual Meeting, Pittsburgh, accepted for Platform Presentation, 2012.
  • Goh SK, Bertera S, Olsen P, Banerjee I. Perfusion-decellularized Pancreas as a Scaffold for the Differentiation of Human Embryonic Stem Cells into Insulin-Producing Cells. International Society for Stem Cells Research (ISSCR) 10 Annual Meeting, Yokohama, Japan – International Meeting, accepted for Platform Presentation, 2012.
  • Goh SK, Bertera S, Olsen P, Banerjee I. Perfusion-decellularized Pancreas as a Scaffold for the
    Differentiation of Human Embryonic Stem Cells into Insulin-Producing Cells. 7 Biologic Scaffold Symposium, Napa Valley, accepted for Platform Presentation, 2012.
  • Goh SK, Bertera S, Banerjee I. Perfusion-decellularization of Pancreatic Matrix- A Scaffold for Bio-engineered Pancreas. BMES Annual Meeting, Hartford, accepted for Platform Presentation, 2011.
  • Goh SK, Bertera S, Banerjee I. Perfusion-decellularization of Pancreatic Matrix- A Scaffold for Bio-engineered Pancreas. AIChE Annual Meeting, Minneapolis, accepted for Platform Presentation, 2011.