1178A, Benedum Hall
My research is focused on investigating the potential use of native-derived whole organ scaffold as a biomaterial candidate to support pancreatic differentiation of embryonic stem cells. Our long-term goal is to design and show proof of concept of a regenerative medicine strategy that reconstructs functional insulin secreting cells for treatment of diabetes. Recently described concept of whole organ engineering provides a possible avenue by which functional endocrine pancreatic tissue can be created. By using the native pancreatic extracellular matrix as a favorable 3-D microenvironmental niche and combining this 3-D scaffold with relevant cell types, there is a potential that a functional endocrine pancreas can be “manufactured” for therapeutic use. While the importance of native microenvironment in organ functionality is well appreciated, the required extent of specificity of this native microenvironment is still an outstanding question.
Specific aims to support our long-term goal are:
Generation and characterization of perfusion-decellularized whole organ pancreas.
Repopulation and differentiation of hESC in 3D whole organ pancreatic microenvironment
Solubilization of whole organ-derived ECM to evaluate matrix specificity and cell-ECM interaction during pancreatic differentiation
2012 Teaching Assistant: Bioengineering class - BIOENG2521, Molecular Cell Biology and Biophysics I at University of Pittsburgh taught by Dr. Partha Roy. 2011 Teaching Assistant: Bioengineering class - BIOENG2520, Molecular Cell Biology and Biophysics II at University of Pittsburgh taught by Dr. Partha Roy.