Pitt | Swanson Engineering
Joe Candiello


 

1178A, Benedum Hall

jec40@pitt.edu

Link to CV

                                                

 

Research Focus 

Stem cells receive both chemical and mechanical cues to help drive differentiation. The investigation into the biophysical communication between embryonic stem cells and their immediate microenvironment cues will allow us to tailor materials to maximize the response of ESCs towards a desired end cell phenotype. Specifically we are investigating if mechanical stimuli can be one of many factors to facilitate differentiation of hESCs towards mature insulin producing cell for the treatment of diabetes. Beyond just mechanical stimulation, we are also investigating whether specific organic or inorganic materials have a biophysical effect on downstream ESC lineage fate, which may be used as part of a larger multi-faceted differentiation approach. Additionally, we are determining a quantitative method to track cytoskeleton development during ESC differentiation and the possible downstream implications on mature cellular function.

Education  

PhD, Bioengineering, University of Pittsburgh, 2009
Thesis Topic: Investigation into the Biomechanical Properties or Basement Membranes by Atomic Force Microscopy

BS, Engineering Science and Mechanics, Pennsylvania State University, 2002
 
Publications  
  • “Early differentiation of mouse embryonic stem cells in response to variations in alginate substrate stiffness.” Joseph E Candiello, Singh SS, Task K, Kumta PN, Banerjee I. J Biol Eng, 2013, Apr 9; 7(1):9
  • “Role of substrates in diabetes therapy: stem cell differentiation and islet transplantation.” Joseph E Candiello, Jaramillo M, Goh SK, Banerjee I. Crit Rev Biomed Eng. 2011; 39 (6):535-55
  • “Age-dependent changes in the structure, composition, and biophysical properties of a human basement membrane.” Joseph E Candiello, Cole GJ, Halfter W. Matrix Biol. 2010 Jun; 29(5):402-10
  • “Biochemical and biophysical changes underlie the mechanisms of basement membrane disruptions in a mouse model of dystroglycanopathy.” Zhang P, Yang Y, Candiello JE, Thorn TL, Gray N, Halfter WM, Hu H. Matrix Biol. 2013 Feb 27
  • “Biomechanical properties of native basement membranes.” Joseph E Candiello, Balasubramani M, Schreiber EM, Cole GJ, Mayer U, Halfter W, Lin H.
  • “Protein composition and biomechanical properties of in vivo-derived basement membranes.” Halfter W, Candiello JE, Hu H, Zhang P, Schreiber E, Balasubramani M. Cell Adh Migr. 2013 Jan-Feb; 7(1): 64-71
  • “Whole-cell electrical activity under direct mechanical stimulus by AFM cantilever using planar patch clamp chip approach.” Upadhye KV, Candiello JE, Davidson LA, Lin H. Cell Mol Bioeng. 2011 Jun; 4(2):270-280
  • “Sensing and modulation of invadopodia across a wide range of rigidities.” Parekh A, Ruppender NS, Branch KM, Sewell-Loftin MK, Lin J, Boyer PD, Candiello JE, Merryman WD, Guelcher SA, Weaver AM. Biophys J. 2011 Feb 2; 100(3):573-82
  • “Retinal ectopias and mechanically weakened basement membrane in a mouse model of muscle-eye-brain (MEB) disease congenital muscular dystrophy.” Hu H, Candiello JE, Zhang P, Ball SL, Cameron DA, Halfter W. Mol Vis 2010 Jul 28; 16:1415-28
  • “Molecular interactions in the retinal basement membrane system: a proteomic approach.” Balasubramani M, Schreiber EM, Candiello JE, Balasubramani GK, Kurtz J, Halfter W. Matrix Biol. 2010 Jul, 29(6): 471-83
  • “Differences in tissue-remodeling potential of aortic and pulmonary heart valve interstitial cells.” Merryman WD, Liao J, Parekh A, Candiello JE, Lin H, Sacks MS. Tissue Eng. 2007 Sep. 13 (9):22881-9

Conference Presentations 

  • “Mouse Embryonic Stem Cell Sensitivity to Compliant Alginate Substrates: A Promising Platform for Endoderm Leaning Differentiation” Joseph E Candiello, Singh S, Kumta P, Banerjee I. AIChE Annual Meeting, 2012, Oct 30, #279059
  • “Alginate encapsulated activinA doped chitosan nanoparticles (CNP) to promote endodermal differentiation in human embryonic stem cells.” Joseph E Candiello, Richardson T, Kumta P, Banerjee I. AIChE Annual Meeting, 2012, Oct 30, #279284
  • “Exploring structural variations of cytoskeleton to understand hESC differentiation to insulin producing phenotype.” Joseph E Candiello, Zhang LA, Kumta P, Banerjee I. AIChE Annual Meeting, 2012, Nov 1, #279665
  • “Atomic force microscopy of the anterior lens capsule in eyes with exfoliation syndrome” Candiello JE, Ritch R, Lin H. Invest. Ophthalmol. Vis. Sci 48: E-abstract 1201. ARVO conference 2007
  • “Changes of structural and biomechanical properties of inner limiting membrane during development.” Candiello JE, Halfter W, Lin H. Poster presented to the Biomedical Engineering Society, Chicago, Ill. 2006
  • “Biomechanical propertied of the inner limiting membrane of the embryonic retina” Lin H, Candiello JE, Halfter W. Invest. Ophthalmol. Vis. Sci 47: E-abstract 2786. ARVO conference 2006
  • “Calcification induced changes in Bruch’s membrane elasticity.” Candiello JE, Feola A, Friberg TR, Lin H. Invest. Ophthalmol. Vis. Sci 47: E-abstract 1398. ARVO conference 2006
  • “Biomechanical propertied of the Bruch’s membrane: an atomic force microscopy study.” Candiello JE, Elyaderani A, Feola AJ, Friberg TR, Lin H. Invest. Ophthalmol. Vis. Sci 46: E-abstract 1210. ARVO conference 2005. Paper presented to panel on age-related macular degeneration.
  • “Mapping of the Bruch’s membrane elasticity.” Candiello JE, Elyaderani A, Feola AJ, Friberg TR, Lin H. Paper/Talk presented to the Biomedical Engineering Society, Philadelphia, PA 2004