Pitt | Swanson Engineering
Characterization of Vascular Collagen and Elastin in Abdominal Aortic Aneurysm by Fourier Transform Infrared Imaging

Progressive destruction of the aortic extracellular matrix is a common feature of aortic aneurysm. In collaboration with Dr. Nancy Pleshko at Temple University, we have studied the distribution of two key extracellular matrix components, collagen and elastin, in human aneurysm biopsies. Dr. Pleshko’s technology centers on the use of Fourier Transform Infrared Imaging Spectroscopy. In this technique, the interaction of molecular bonds with varying wavelengths of infrared light is measured and molecular structures can be characterized based on signature spectral absorbance bands. The second derivative of the spectra can increase the ability to detect specific absorption bands by resolving overlapping bands in raw spectra. By studying these second derivative spectra, we were able to distinguish ECM components in the AAA wall samples, consistent with histochemically stained images. Spectroscopic imaging technology has the potential for translation to a clinical environment to examine ECM changes in a minimally invasive fashion using fiber optic technology, and this study will lay the groundwork for future in vivo clinical studies.

References

Infrared Spectroscopy to Map Extracellular Components in Abdominal Aortic Aneurysm Wall – Rabee Cheheltani, Jayashree Rao, David A. Vorp, Mohammad F. Kiani, Nancy Pleshko: BMES 2012, Atlanta

 

Related publications

1.    Cheheltani R, McGoverin CM, Rao J, Vorp DA, Kiani MF, Pleshko N, “Fourier transform infrared   spectroscopy to quantify collagen and elastin in an in vitro model of extracellular matrix degradation in  aorta”, Analyst 2014 Jun 21;139(12):3039-47. PMID 24761431, PMCID: PMC4096121