Progressive destruction of the
aortic extracellular matrix is
a common feature of aortic aneurysm. In collaboration with Dr. Nancy Pleshko at
Temple University, we have studied the distribution of two key extracellular
matrix components, collagen and elastin, in human aneurysm biopsies. Dr.
Pleshko’s technology centers on the use of Fourier Transform Infrared Imaging Spectroscopy. In this
technique, the interaction of molecular bonds with varying wavelengths of infrared light is measured and molecular structures can be characterized based on signature spectral absorbance bands.
The second derivative of the spectra can increase the ability to detect specific absorption bands by
resolving overlapping bands in raw spectra.
By studying these second derivative spectra, we were able to distinguish ECM components in the AAA wall samples, consistent with histochemically stained
imaging technology has the potential for translation to a clinical environment to examine ECM changes in a minimally invasive fashion using fiber optic technology, and this study will lay the groundwork for
future in vivo clinical studies.
Infrared Spectroscopy to Map Extracellular Components in Abdominal Aortic Aneurysm Wall – Rabee
Cheheltani, Jayashree Rao, David A. Vorp, Mohammad F. Kiani, Nancy Pleshko: BMES 2012, Atlanta
R, McGoverin CM, Rao J, Vorp DA,
Kiani MF, Pleshko N, “Fourier transform infrared spectroscopy to quantify collagen and
elastin in an in vitro model of extracellular matrix degradation in aorta”, Analyst 2014 Jun 21;139(12):3039-47.
PMID 24761431, PMCID: PMC4096121