Pitt | Swanson Engineering
Ataai, Mohammad M
Chem/Petroleum Engineering, Bioengineering Department
Ataai, Mohammad M
Faculty
Professor
Office: 908 Benedum Hall
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Ph.D. in Chemical Engineering, Cornell University, 1986

M.S. in Chemical Engineering, Tufts University, 1981

Cell Culture

Metabolism and Fermentation

Protein Purification

Phalakornkule, C., Lee, S., Zhu, T., Koepsel, R., Ataai, M.M., Grossmann, I.E., and Domach, M.M., In press, "A MILP-Based flux alternative generation & NMR experimental design strategy for metabolic engineering," Metabolic Engineering.

Lee, J., Goel, A., Ataai, M.M., and Domach, M.M., 1997, "optimality of co-feeding of carbon sources for maximizing cellular and energetic yields using a constrained network analysis," Appl Environ. Microbiol., vol.63, pp. 710-718, Manuscript submitted for publication.

Bartlow, P., Tiwari, N., Beitle, R.R., and Ataai, M.M., 2012, "Evaluation of Escherichia coli proteins that burden nonaffinity-based chromatography as a potential strategy for improved purification performance," Biotech Prog., vol.28, pp. 137-145.

Meade, J., Khan, S., Ataai, M.M., and Domach, M.M., 2012, "Use of Flux Pre-Analysis to Enable 13-C Tracer Studies in Pyruvate Kinase-Deficient Escherichia coli," Biotechnol. J., vol.7, pp. 449-460.

Bartlow, P., Uechi, G.T., Cardamone, J.J., Sultana, T., Fruchtl, M., Beitlee, R.R., and Ataai, M.M., 2011, "Identification of native Escherichia coli BL21 (DE3) proteins that bind to immobilized metal affinity chromatography under high imidazole conditions and use of 2D-DIGE to evaluate contamination pools with respect to recombinant protein expression lev," Protein Expression and Purification, vol.78, no.2, pp. 216-224.

Pan, Z., Cunningham, D.S., Zhu, T., Ye, K., Koepsel, R.R., Domach, M.M., and Ataai, M.M., 2010, "Enhanced recombinant protein production in pyk mutant of Bacillus subtilis," Appl Microbiology Biotechnol., vol.85, pp. 1769-1778.

Tiwari, N., Woods, L., Haley, R., Kight, A., Goforth, R., Clark, K., Ataai, M.M., and Beitle, R., 2010, "Identification and characterization of native proteins of Escherichia coli BL-21 that display affinity towards Immobilized Metal Affinity Chromatography and Hydrophobic Interaction Chromatography Matrices," Protein Expression Purification, vol.70, pp. 191-195.

Cunningham, D., Koepsel, R.R., Ataai, M.M., and Domach, M.M., 2009, "Factors Affecting Plasmid Production in Escherichia coli from a Resource Allocation Standpoint," Microbial Cell Factory, vol.8, pp. 1-17.

Cunningham, D., Liu, Z., Domagalski, N., Koepsel, R.R., Ataai, M.M., and Domach, M.M., 2009, "Pyruvate Kinase Deficient Escherichia coli Exhibits Increased Plasmid Copy Number and Cyclic AMP," J. Bacteriol., vol.191, no.9, pp. 3041-3049.

Dilmore, R.M., Griffith, C., Liu, Z., Soong, Y., Hedges, S.W., Koepsel, R.R., and Ataai, M.M., 2009, "Carbonic anhydrase-facilitated CO2 absorption with polyacrylamide buffering bead capture," with polyacrylamide buffering bead capture, International Journal of Greenhouse Gas Control, vol.2, pp. 401-410.

Liu, Z., Bartlow, P., Dilmore, R.M., Soong, Y., Pan, Z., Koepsel, R.R., and Ataai, M.M., 2009, "Production, Purification and Characterization of a Fusion Protein of Carbonic Anhydrase from Neisseria gonorrhoeae and Cellulose Binding Domain from Clostridium thermocellum," Biotech Prog, vol.25, pp. 68-74.

Liu, Z., Patrick, B., Varakala, R., Beitle, R.R., Koepsel, R.R., and Ataai, M.M., 2009, "Use of proteomics for design of a tailored host cell for highly efficient protein purification," J. Chromatography A, vol.1216, pp. 2433-2438.

Grant, K.G., Krisky, D.M., Ataai, M.M., and Glorioso, J.C., 2008, "Engineering cell lines for production of replication defective HSV-1 gene therapy vectors.," Biotechnol & Bioeng., vol.102, no.4, pp. 1087-1097.

Ghosh, T., Grossmann, I.E., Ataai, M.M., and Domach, M.M., 2006, "A Three-Level Problem-Centric Strategy for Selecting NMR Precursor Labeling & Analytes," Metabolic Engineering, vol.8, pp. 491-507.

Jiang, C., Ataai, M.M., Ozuer, A., Krisky, D., Wechuck, J., Pornsuwan, S., Pourarian, F., and Glorioso, J.C., 2006, "Inactivation of Herpes Simplex Type 1 Gene Vector on Immobilized Metal Affinity Chromatography: Oxidative Damage by Hydroxyl Free Radicals and its Prevention," Biotechnol. & Bioeng., vol.95, pp. 48-57.

Jiang, C., Gloioso, J.C., and Ataai, M.M., 2006, "Presence of Imidazole in Loading Buffer Prevents Formation of Free Radical in IMAC and Dramatically Improves the Recovery of HSV-1 Gene Therapy Vectors," J. Chromatography A, J Chromatogr A, vol.1121, no.1, pp. 40-45.

Pan, Z., Zhu, T., Domagalski, N., Khan, S., Domach, M.M., and Ataai, M.M., 2006, "Regulated Expression of Pyruvate Kinase in B. subtilis for Control of growth rate and Formation of Acidic By-products," Biotechnol. Prog., vol.22, pp. 1451-1455.

Zhu, T., Pan, Z., Domagalski, N., Koepsel, R.R., Ataai, M.M., and Domach, M.M., 2005, "Engineering of Bacillus subtilis for enhanced total synthesis of folic acid," Appl Environ Microbiol, vol.71, pp. 7122-7129.

Canping, J., Wechuck, J.B., Goins, W.F., Krisky, D.M., Wolfe, D., Ataai, M.M., and Glorioso, J.C., 2004, "Immobilized cobalt affinity chromatography provides a novel, efficient method for HSV-1 gene vector purification," J. Virol., vol.78, pp. 8994-9006.

Ghosh, S., Zhu, T., Grossmann, I.E., Ataai, M.M., and Domach, M.M., 2004, "Closing the loop between feasible flux scenario identification for construct evaluation and resolution of realized fluxes via NMR," Comput. Chem. Eng, vol.29, no.3, pp. 459-466.

Ye, K., Jin, S., Ataai, M.M., and Schultz, S., 2004, "Tagging retrovirus with a metal binding peptide: a novel approach for preparation of clinical-grade retroviral vectors by immobilized metal affinity chromatography purification," J. Virol., vol.78, pp. 9820-9827.

Zhu, T., Ataai, M.M., Domach, M.M., and Grossmann, I.E., 2003, "A metabolic network analysis & NMR experimental design program with user interface-driven model construction," Metabolic Engineering, vol.5, pp. 74-85.

Zhu, T., Grossmann, I.E., Domach, M.M., and Ataai, M.M., 2003, "Theoretical improvement of folic acid production in E. coli," Fermentation Biotechnology, ACS symposium series, vol.862, pp. 207-219.

Lee, J., Goel, A., Ataai, M.M., and Domach, M.M., 2002, "Flux Regulation Patterns and Energy Audit of E. coli B/r and K-12," J. Microbiol. Biotechnol., vol.12, pp. 258-267.

Ozuer, A., Wechuck, J.B., Russell, B., Wolfe, D., Goins, W.F., Glorioso, J.C., and Ataai, M.M., 2002, "Evaluation of Infection Parameters in the Production of HSV-1 Viral Vectors For Gene Therapy," Biotech. Prog., vol.18, pp. 476-482.

Ozuer, A., Wetchuck, J.C., Goins, W.F., Wolfe, D., Glorioso, J.C., and Ataai, M.M., 2002, "Effect of Genetic Background, Culture Conditions, and Reactor Configuration on Productivity of HSV-1 Vectors," Biotechnol. Bioeng., vol.77, pp. 685-692.

Wechuck, J.B., Ozuer, A., Goins, W.F., Wolfe, D., Oligino, T., Glorioso, J.C., and Ataai, M.M., 2002, "Effect of Temperature, Medium Composition, and Cell Passage on the Production of Herpes-Based Viral Vectors," Biotechnol. Bioeng., vol.79, pp. 112-119.

Phalakornkule, C., Lee, S., Zhu, T., Koepsel, R., Ataai, M.M., Grossmann, I.E., and Domach, M.M., 2001, "A MILP-Based flux alternative generation & NMR experimental design strategy for metabolic engineering," Metabolic Engineering, vol.3, pp. 124-137.

Ward, M.S., Ataai, M.M., Koepsel, R.R., and Shepherd, R.E., 2001, "Comparison of energy-minimized structures of [PdII(N-methyliminodiacetate)] complexes of X1-His-X3-His-His peptides as an analysis of steric and specific interactions with synthetic binding tags for IMAC separations," Biotechnol. Prog., vol.17, pp. 712-719.

Zhu, T., Phalakornkule, C., Koepsel, R., Domcah, M.M., and Ataai, M.M., 2001, "Cell growth and by-product-Formation in a pyruvate kinase mutant of E. coli," Biotech Progress, vol.17, pp. 624-628.

Chen, Y., Pasuinelli, R., Ataai, M.M., Koepsel, R.R., Kortes, R.A., and Shepherd, R.E., 2000, "Coordination of Two High Affinity Six-mer Peptides to CuII and PdII Models of the Peptide-Metal Chelation Site on IMAC Resins," Inorganic Chemistry, vol.39, pp. 1180-1186.

Fry, B., Zhu, T., Phalakornkule, C., Koepsel, R., Domach, M.M., and Ataai, M.M., 2000, "Characterization of Growth and Acid Formation in a Bacillus subtilis Pyruvate Kinase mutant," Appl Environ. Microbiol, vol.66, pp. 1045-1049.

Lee, S., Phalakornkule, C., Ataai, M.M., Domach, M.M., and Grossmann, I.E., 2000, "Recursive MILP Model for Finding All the Alternate Optima in LP Models for Metabolic Networks," Computers in Chemical Engineering, vol.39, pp. 1180-1186.

Pasquinelli, R.S., Zhao, A., Shepherd, R.E., Koepsel, R.R., and Ataai, M.M., 2000, "Design of Affinity Tags for One Step Protein Purification from Immobilized Zinc Columns," Biotech. Prog., vol.16, pp. 86-91.

Phalakornkule, C., Fry, B., Koepsel, R., Ataai, M.M., and Domach, M.M., 2000, "13C-NMR evidence for pyruvate kinase flux attenuation underlying suppressed acid formation in B. subtilis cultures," Biotech Prog., vol.16, pp. 169-175.

Wetchuck, J.C., Goins, W.F., and Ataai, M.M., 2000, "Effect of Protease Inhibitors on Yield of HSV-1-Based Viral Vectors," Biotech. Progress, vol.16, pp. 493-496.

Wu, N., and Ataai, M.M., 2000, "Production of Viral Vectors for Gene Therapy Applications," Curr. Opin. Biotech., vol.11, no.2, pp. 205-208.

Chen, Y., Pasquinelli, R., Ataai, M.M., Koepself, R.R., Stringfield, T.W., and Shepherd, R.E., 1999, "Coordination of the Six-mer Peptide Gly-His-Pro-His-His-Gly to CuII and PdII N-Methyliminodiacetate Complexes as IMAC Chelation Site Models," Inorganic Biochem., vol.76, pp. 211-220.

Goel, A., Domach, M.M., and Ataai, M.M., 1999, "Metabolite fluxes, pools and enzyme measurements suggest a tighter coupling of the energetic and biosynthetic reactions requires reduction in pyruvate kinase flux," Biotechnol Bioeng., vol.64, pp. 129-134.

Pasquinelli, R.S., Wu, N., Koepsel, R.R., and Ataai, M.M., 1999, "Vector Engineering Anomalies: Impact on Fusion Protein Purification," Protein Expression & Purification, vol.17, pp. 449-455.

Patwardhan, A., Pascquinelli, R., Koepsel, R., and Ataai, M.M., 1998, "Phage-displayed libraries for the selection of optimal affinity tails for Ni-NTA purification of recombinant proteins," Biotechnology Techniques, vol.12, pp. 421-424.

Bhatia, R., Jesionowski, G., and Ataai, M.M., 1997, "Insect cell physiology," Cytotechnology, vol.24, pp. 1-9.

Goud, N., Patwardhan, A., Koepsel, R., and Ataai, M.M., 1997, "Selection of specific peptide ligands for Immobilized Cu (II) using a phage displayed peptide library on bacteriophage," Intl. J. Biochromatography, vol.3, pp. 123-136.

Jesionowski, G., and Ataai, M.M., 1997, "Drastic improvements in insect cell/reccombinat baculovirus protein productivity cultured in a physiologically designed medium," Biotechnology Progress, vol.13, pp. 355-360.

Patwardhan, A., Goud, N., Koepsel, R., and Ataai, M.M., 1997, "Selection of optimum affinity tags from a phage displayed peptide library: Application to immobilized copper affinity chromatography," J. Chromatography, vol.787, pp. 91-100.

Patwardhan, A.V., and Ataai, M.M., 1997, "Mathematical modeling of protein purification of E. coli cell extract using immobilized metal affinity chromatography: Effect of loading pH on the column capacity and purity of the target protein," AIChE J, vol.43, pp. 3232-3240.

Patwardhan, A.V., and Ataai, M.M., 1997, "Site accessibility and the pH dependence of the saturation capacity of a highly cross-linked matrix: Immobilized Metal Affinity Chromatography of BSA on chelating Superose," J. Chromatography, vol.767, pp. 11-23.

Wagner, W.R., Muzzio, D.J., Rilo, H.R., Deglaus, T., and Ataai, M.M., 1997, "Effect of Growth Factors and Defined Medium on Primary Hepatocyte Culture on Polyester Carriers With Varying Surface Treatments," Tissue Engineering, vol.3, pp. 298-301.

Beitle, R.R., and Ataai, M.M., 1996, "Immobilization of enzymes in chelated-metal support," in Methods In Molecular Biotechnology, Eds, 1, G.F. Bickerstaff, ed., pp. 339-343.

Bhatia, R., Jesionowski, G., and Ataai, M.M., 1996, "Insect cell physiology," in Insect Cell Cultures: Fundamental and Applied Aspects, E. Vlak, ed., pp. 33-41.

Goel, A., Domach, M.M., and Ataai, M.M., 1996, "coordination of glycolysis and TCA cycle reaction networks: citrate-glucose cometabolism eliminates acids and reveals potential metabolic engineering strategies," Annals. NY Acad. Sci., vol.782, pp. 1-16.

Parwardhan, A.V., and Ataai, M.M., 1996, "One step purification of human interleukin 1b using IMAC," J. Biochromatography, vol.2, pp. 119-124.

Goel, A., Lee, J.W., Domach, M.M., and Ataai, M.M., 1995, "suppressed acid formation by cofeeding glucose and citrate in bacillus cultures: emergence of pyruvate kinase as a potential metabolic engineering site," Biotech. Prog., vol.11, pp. 380-386.

Parwardhan, A.V., Zenouzi, M., and Ataai, M.M., 1995, "A mathematical model of immobilized metal affinity chromatography: Simulation of loading and elution conditions," ASME Proceedings, vol.32, pp. 61-68.

Parwardhan, A.V., Zenouzi, M., and Ataai, M.M., 1995, "Design of efficient loading and elution strategies for IMAC separations: purification of human interleukin 1B from E. coli cell extract," AIChE Proceedings On Recent Development and Future Opportunities In Separation Technology, vol.1, pp. 418-424.

Vierheller, C., Goal, A., Domach, M.M., and Ataai, M.M., 1995, "Sustained and constitutive high levels of protein production in continuous cultures of B. subtilis," Biotechnol. Bioeng., vol.47, pp. 520-524.

Lee, J., Goel, A., Ataai, M.M., and Domach, M.M., 1994, "Flux adaptation of a citrate synthase mutant of E. coli," Ann. NY. Acad. Sci., vol.745, pp. 35-59.

Almedia, N., Beckman, E.J., and Ataai, M.M., 1993, "Immobilization of glucose oxidase in thin polypyrrole films: influence of polymerization conditions and film thickness on activity and stability of immobilized enzyme," Biotechnol. Bioeng, vol.42, pp. 1037-1045.

Beitle, R.R., and Ataai, M.M., 1993, "One step purification to homogeneity of a model protein from inclusion bodies by its fusion to an effective metal-binding peptide," Biotechnol. Prog., vol.9, pp. 64-69.

Beitle, R.R., Sagar, S.L., Ataai, M.M., and Domach, M.M., 1993, "Metal-based affinity separation of a- and b-chymotrypsin," Bioseparation, vol.3, pp. 291-296.

Ferrance, J., Goel, A., and Ataai, M.M., 1993, "Utilization of glucose & amino acids in insect cell cultures: quantifying the metabolic flows within the primary pathways and medium development," Biotechnol. Bioeng., vol.42, pp. 697-707.

Goel, A., Ferrance, J., and Ataai, M.M., 1993, "analysis of metabolic fluxes in batch and continuous cultures of Bacillus Subtilis," Biotechnol. Bioeng., vol.42, pp. 686-696.

Beitle, R.R., and Ataai, M.M., 1992, "Highly efficient purification of proteins extended by a naturally occurring metal binding peptide from cell extracts and inclusion body proteins," Symposium On Separation Technologies, AIChE, pp. 1001-1004.

Beitle, R.R., and Ataai, M.M., 1992, "Immobilized metal affinity chromatography and related techniques," in Developments In Bioseparation, M.M. Ataai, and S. Sikdar, eds., AIChE, pp. 48-60.

Beitle, R.R., and Ataai, M.M., 1991, "A unique monitoring and control system for laboratory fermenters," Biotechnology Techniques, vol.5, pp. 77-82.

Beitle, R.R., and Ataai, M.M., 1991, "Purification of TEM-b-lactamase from cell extract using immobilized copper affinity chromatography," Biotechnology Techniques, vol.5, pp. 107-110.

Jeong, J.W., and Ataai, M.M., 1990, "A Highly Structured Model for Simulation of Batch and Continuous Culture of B. subtilis and Examination of Cellular Differentiation," Ann. NY. Acad. Sci., vol.589, pp. 82-90.

Jeong, J.W., Snay, J., and Ataai, M.M., 1990, "A Model For Examining Growth and Sporulation Processes of B. subtilis," Biotechnol. Bioeng., vol.35, pp. 160-184.

Ataai, M.M., and Jachowicz, J., 1989, "RepA-mRNA a Potential Rate-Limiting Factor in Replication of R1 Plasmids," in Frontiers in Bioprocessing, S.K. Sikdar, ed., CRC Press, pp. 159-165.

Ataai, M.M., Strohm, W., and Jeong, J.W., 1989, "Protease Problem in Relation to B. subtilis as a Host of Recombinant Vectors," in Frontiers in Bioprocessing, S.K. Sikdar, ed., CRC Press, pp. 167-172.

Shuler, M.L., 1989, "Kinetics of Enzyme Catalyzed Reactions," in Chemical Engineering Problems in Biotechnology, M.M. Ataai, ed., AIChE, pp. 207-230.

Snay, J., Jeong, J., and Ataai, M.M., 1989, "Effect of growth Conditions on Carbon Utilization and Organic By-Product Formation," Biotechnol. Prog., vol.5, pp. 63-69.

Ataai, M.M., and Shuler, M.L., 1988, "Growth Behavior and Prediction of Copy Number and Retention of ColE1 Type Plasmids in E. coli Under Slow Growth Conditions," in Handbook of anaerobic Fermentation, L.E. Erickson, and D.Y. Fung, eds., Marcel Decker, New York.

Longo, A., Snay, J., Zimmer, L., and Ataai, M.M., 1988, "Calcium Dependence of Proteolytic Activity of Residual Protease of B. subtilis," Biotechnology Letters, vol.10, pp. 649-654.

Ataai, M.M., and Shuler, M.L., 1987, "A Mathematical Model for Prediction of Plasmid Copy Number and Genetic Stability in E. coli,," Biotechnol. Bioeng., vol.30, no.389-397.

Kim, B., Good, T.A., Ataai, M.M., and Shuler, M.L., 1987, "Growth Behavior and Prediction of Copy Number and Retention of ColE1 Type Plasmids in E. coli Under Slow Growth Conditions," Ann. N.Y. Acad. Sci., vol.506, pp. 384-395.

Ataai, M.M., and Shuler, M.L., 1986, "A Mathematical Model for Control of ColE1 Type Plasmid Replication," Plasmid, vol.16, pp. 204-212.

Ataai, M.M., and Shuler, M.L., 1985, "Simulation of CFSTR Through Development of a Mathematical Model for Anaerobic Growth of E. coli Cell Population," Biotechnol. Bioeng., vol.27, pp. 1051-1055.

Ataai, M.M., and Shuler, M.L., 1985, "Simulation of the Growth Pattern of a Single Cell of E. coli Under Anaerobic Conditions," Biotechnol. Bioeng., vol.27, pp. 1027-1035.

Lee, A., Ataai, M.M., and Shuler, M.L., 1984, "Double substrate Limited Growth of Escherichia coli," Biotechnol. Bioeng., vol.26, pp. 1398-1401.

Frohlich, B.T., Webster, I.A., Ataai, M.M., and Shuler, M.L., 1983, "Photobioreactors: Models for Interaction of Light Intensity, Reactor Design, and Algal Physiology," Biotechnol. Bioeng. Symp., vol.13, pp. 331-350.

Bartlow, P.R., Tiwari, N., Beitle, R.R., and Ataai, M.M., 2011, "Evaluation of Escherichia Coli Proteins That Burden Recombinant Protein Purification On Inexpensive, Non-Affinity-Based Resins," AIChE Annual Meeting, Minneapolis, MN.

Brune, E.M., Rechtin, T.M., Kight, A., Ataai, M.M., Henry, R., and Beitle, R.R., 2011, "Proteome-Based Development of Affinity Motifs and Reduced Contaminant Strains," AIChE Annual Meeting, Minneapolis, MN.

Trivedi, R.N., Bartlow, P.R., Meade, J., Khan, S.A., Domach, M.M., and Ataai, M.M., 2011, "Highly Improved Plasmid DNA Production by Optimizing the Plasmid Construct and Fermentation Conditions," AIChE Annual Meeting, Minneapolis, MN.

Bartlow, P., Haley, R., Beitle, R.R., and Ataai, M.M., 2009, "Knockout and Mutational Tailoring of Host Strains to Simplify Bioseparation," AIChE Annual Meeting, Nashville, Tennessee.

Cunningham, D.S., Khan, S.A., Ataai, M.M., and Domach, M.M., 2009, "Strain design for maximizing the yield of therapeutic DNA," American Institute of Chemical Engineers Society for Biological Engineers 2nd International Conference on Accelerating Biopharmaceutical Development, Coronado, CA.

Cunningham, D.S., Shan, S.A., Ataai, M.M., and Domach, M.M., 2009, "Metabolic engineering for increasing plasmid yield," American Chemical Society 238th National Meeting & Exposition, Washington, DC.

Tiwari, N., Kight, A., Woods, L., Haley, R., Goforth, R., Clark, K., Henry, R., Ataai, M.M., and Beitle, R.R., 2009, "Identification and characterization of native proteins of Escherichia coli BL-21 that display affinity towards Immobilized Metal Affinity Chromatography and Hydrophobic Interaction Chromatography Matrices," AIChE Annual Meeting, Nashville, Tennessee.

Liu, Z., Bartlow, P., Varakala, R., Beitle, R.R., Koepsel, R.R., and Ataai, M.M., 2008, "Design of a Tailored Host Cell for Highly Efficient Protein Purification," American Chemical Society, National Meeting & Exposition Division of Biotechnology, Philadelphia, PA.

Cunningham, D.S., Domagalski, N., Koepsel, R., Ataai, M.M., and Domach, M.M., 2007, "Probing the supply-side attributes of different metabolically engineered host backgrounds," American Chemical Society 234th National Meeting & Exposition, Boston, MA.

Grant, K.G., Drisky, D.M., Ataai, M.M., and Glorioso, J.C., 2007, "Engineering cell lines for the production of highly replication defective HSV-1 vectors," International Herpesvirus Workshop, Asheville, NC.

Haley, R., Varakala, R., Goforth, R.L., Ataai, M.M., Henry, R., and Beitle, R.R., 2007, "Bio-Separations Process Improvement via Genomic Manipulation," Cambridge Health Institute Conference Series, San Diego.

Liu, Z., Bartlow, P., Dilmore, R.M., Soong, Y., Pan, Z., Koepsel, R.R., and Ataai, M.M., 2007, "Production, Purification and Characterization of a Fusion Protein of Carbonic Anhydrase from Neisseria gonorrhoeae and Cellulose Binding Domain from Clostridium thermocellum," AIChE mtg, Salt Lake City, Utah.

Varakala, R., Haley, R., Beitle, R.R., and Ataai, M.M., 2007, "Proteomic characterization of E. coli metal binding proteins," ACS National Meeting, Boston.

Varakala, R., Haley, R., Henry, R., Ataai, M.M., and Beitle, R.R., 2007, "Profiling metal binding E. coli proteins: Its use in purification process development," ACS Meeting, Boston.

Varakala, R., Haley, R., Henry, R., Ataai, M.M., and Beitle, R.R., 2007, "The Metalloproteome of Escherichia coli: Its Application in Downstream Processing Via IMAC," AIChE Annual Meeting.

Beitle, R.R., Varakala, R., Haley, R., Henry, A., and Ataai, M.M., 2006, "A Rational Method to Improve Bioseparation Via Proteomics," American Institute of Chemical Engineers, San Francisco.

Ghosh, S., Grossmann, I.E., Ataai, M.M., and Domach, M.M., 2006, "Three Level Screening Strategy for Effectively Using NMR to Identify Fluxes," ACS Annual Mtg, San Francisco, CA.

Grant, K., Krisky, D., Ataai, M.M., and Glorioso, J.C., 2006, "Cell line engineering for the production of high replication defective HSV-1 vector," American Society of Gene Therapy, Baltimore, MD.

Varakala, R., Haley, R., Koepsel, R., Ataai, M.M., Mohammad, M., and Beitle, R.R., 2006, "Development of a general process for minimization of genomic contaminants: Chromatographic and metabolic considerations," ACS National Meeting, San Francisco.

Zhiwei, P., Zhu, T., Koepsel, R., Domach, M.M., and Ataai, M.M., 2006, "Control of Cell Growth and Glucose Uptake Rates by Controlled Expression of Phosphofructokinase (Pfk) in B. Subtilis," American Institute of Chemical Engineers, San Francisco.

Domagalski, N.R., Pan, Z., Zhu, Z.T., Ye, K., Koepsel, R., Ataai, M.M., and Domach, M.M., 2005, "Combining enhanced traits of E. coli into B. subtilis," ACS Natl Mtg, San Diego, CA.

Grant, K.G., Ataai, M.M., Glorioso, J.C., and Krisky, D., 2005, "Cell line development for production of clinical grade HSV vectors," ACS Natl Mtg, San Diego, CA.

Haley, R., Henry, R., Goforth, R., Ataai, M.M., and Beitle, R.R., 2005, "Circumventing the Effects of High Binding Immobilized Metal Affinity Chromatography Contaminants," AICHE 2005 annual meeting, Cincinnati, OH.

Jiang, C., Ataai, M.M., and Glorioso, J.C., 2005, "Inactivation of biological product on immobilized metal affinity chromatography by hydroxyl free radical and methods of prevention," Bioprocess International World Conference and Exhibition, Boston, MA.

Jiang, C., Wechuck, J.B., Krisky, D., Glorioso, J.C., and Ataai, M.M., 2005, "Immobilized cobalt affinity purification for HSV-1 gene therapy vector," ACS Natl Mtg, San Diego, CA.

Jiang, C., Wechuck, J.B., Krisky, D., Glorioso, J.C., and Ataai, M.M., 2005, "Reducing agents and imidazole protect HSV-1 vectors from inactivation during immobilized cobalt affinity chromatography," AICHE 2005 annual meeting, Cincinnati, OH.

Pan, Z.T., Zhu, Z.T., Domagalski, N.R., Koepsel, R., Domach, M.M., and Ataai, M.M., 2005, "Regulated expression of pyk in B. subtilis to reduce formation of acidic by-products and improve production of of folic acid and recombinant protein," ACS Natl Mtg, San Diego, CA.

Ye, K., Jin, S., Ataai, M.M., and Schultz, J., 2005, "One step purification of retrovirus vectors by IMAC," ACS Natl Mtg, San Diego, CA.

Beitle, R., Cai, Y., Varakala, R., Henry, R., and Ataai, M.M., 2004, "Proteome based approach to affinity separations," AIChE National Mtg., Austin, TX.

Domagalski, N., Zhu, T., Koepsel, R., Ataai, M.M., and Domach, M.M., 2004, "Green fluorescent protein expression increased in pyruvate kinase-deficient E. coli," ACS Natl Mtg, Anaheim, CA.

Jiang, C., Krisky, D.M., Goins, W.F., Glorioso, J.C., and Ataai, M.M., 2004, "Developing Vero cell line expressing cobalt affinity peptide tagged HSV-1 gC for delivering the tag on the virus surface facilitating the virus purification," ACS Natl Mtg, Anaheim, CA.

Pan, Z., Zhu, T., Domagalski, N., Koepsel, R., Domach, M.M., and Ataai, M.M., 2004, "Characterization of Growth, intracellular metabolites, by-product formation and recombinant protein production in a regulated pyk background of B. subtilis," AIChE National Mtg., Austin, TX.

Zhu, T., Ataai, M.M., Koepsel, R., and Domach, M.M., 2004, "Metabolic engineering of Bacillus subtilis for enhanced folic acid production," AIChE National Mtg., Austin, TX.

Jiang, C., Wechuck, J.B., Goings, W.F., Krisky, D.M., Glorioso, J.C., and Ataai, M.M., 2003, "A Highly Efficient Purification of HSV-1 Vectors by Immobilized Cobalt Affinity Chromatography," AIChE Annual Natl Mtg, San Francisco CA.

Zhu, T., Domach, M.M., and Ataai, M.M., 2003, "Metabolic Engineering of Folic Acid Production," ACS National Meeting, New Orleans.

Ataai, M.M., 2002, "Production and Purification of Herpes-based viral vectors," Biophex Conference, San Francisco.

Domach, M.M., Zhu, T., Phalakornkule, T., and Ataai, M.M., 2002, "Future Trends in Cellular System Analysis," Annual Meeting, Korean Institute of Chemical Engineers, Plenary Session, Soul, South Korea.

Ozuer, A., Wetchuck, J.C., Goins, W.F., Wolfe, D., Glorioso, J.C., and Ataai, M.M., 2002, "Efficient production and analysis of clinical-grade HSV-1 based gene therapy vectors," ACS National Meeting, Boston, MA.

Ozuer, A., Wetchuck, J.C., Goins, W.F., Wolfe, D., Glorioso, J.C., and Ataai, M.M., 2002, "Production of replication-defective HSV-1 viral vectors and development of rapid and precise assay systems to analyze defective and infective particles," ACS National Meeting, Boston, MA.

Zhu, T., Domach, M.M., and Ataai, M.M., 2002, "Metabolic Engineering of Folic Acid Production," ACS National Meeting, Boston, MA.

Zhu, T., Ghosh, S., Grossmann, I.E., Ataai, M.M., and Domach, M.M., 2002, "Metabologica: A Metabolic Network Analysis & NMR/GC-MS Experiment Design Program," Metabolic Profiling Conference, Durham, NC.

Ozuer, A., Ataai, M.M., and Glorioso, J.C., 2001, "Production of Herpes based Viral Vector and Development of Vector Assay System," Gene Therapy and Vector production, Bethesda, MD.

Ozuer, A., Wechuck, J.B., Goings, W.F., Wolfe, D., Glorioso, J.C., and Ataai, M.M., 2001, "Effect of Genetic Background and Culture Conditions on Production of Herpesvirus-based Gene Therapy Vector," European Society of Gene Therapy, Antalya.

Ozuer, A., Wendell, S., Wolfe, D., Goins, W.F., Ataai, M.M., and Glorioso, J.C., 2001, "Development of Rapid, Accurate and Precise Assay Systems to Analyze The Quality and The Quantity of Clinical Grade Herpes Vector Stocks," American Society of Gene Therapy, Seattle.

Wechuck, J.B., Zhang, X., Wendell, S., Wolfe, D., Goins, W., Greenberger, J., Glorioso, J.C., and Ataai, M.M., 2001, "Gene Transfer to Human Umbilical Cord Blood CD34+ Cells with HSV-1 Viral Vectors," AIChE Natl. Mtg., Reno, NV.

Zhu, T., Domach, M.M., and Ataai, M.M., 2001, "Metabolic network analysis & NMR simulation tools development," AIChE Natl. Mtg., Reno, NV.

Lee, S., Phalakornkule, C., Zhu, T., Ataai, M.M., Domach, M.M., and Grossmann, I.E., 2000, "Recursive MILP Model for Finding All the Alternate Optima in LP Models for Metabolic Networks," ACS Natl Mtg, San Francisco, CA.

Ozuer, A., Wetchuck, J.C., Russell, B., Glorioso, J.C., and Ataai, M.M., 2000, "Production and Characterization of Infective and Defective Herpes Vectors," AIChE Natl Mtg, Los Angeles, CA.

Phalakornkule, C., Zhu, T., Koepsel, R., Domach, M.M., and Ataai, M.M., 2000, "Metabolic flux Analysis Using NMR and Network Analysis," Metabolic Engineering III, Colorado Spring, CO.

Wetchuck, J.C., Glorioso, J.C., and Ataai, M.M., 2000, "Gene Transfer to Human Cord Blood CD34+ Stem Cells. Effects of of Culture Parameters and Genetic Background on Production of HSV1 Viral Vector," AIChE Natl Mtg, Los Angeles, CA.

Zhu, T., Phalakornkule, C., Koepsel, R., Domach, M.M., and Ataai, M.M., 2000, "Software Development for analysis of Reaction Networks," AIChE Natl Mtg, Los Angeles, CA.

Ozuer, A., Wetchuck, J.C., Russell, B., Glorioso, J.C., and Ataai, M.M., 1999, "Effect of temperature, media exchange, and post-infection metabolism on infection of HSV1/Vero Cells," AIChE Natl Mtg, Dallas TX.

Pasquinelli, R., Koepsel, R., and Ataai, M.M., 1999, "Tailoring of fusion peptides for highly efficient separation of recombinant protein on immobilized Zn(II)-IDA," ACS Natl Mtg, CA.

Pasquinelli, R.S., Zhao, A., Shepherd, R.E., Koepsel, R.R., and Ataai, M.M., 1999, "Design of Affinity Tags for One Step Protein Purification," Eng. Foundation Conference, Denver CO.

Wetchuck, J.C., Glorioso, J.C., and Ataai, M.M., 1999, "Efficient Strategies for High Titer Production of Viral Vectors: HSV-1/Vero Cells as a Case Stud," Eng. Foundation Conference, Denver CO.

Zhu, T., Phalakornkule, C., Koepsel, R., Domach, M.M., and Ataai, M.M., 1999, "Metabolic flux analysis using NMR and network analysis growth characterization of the pyruvate kinase mutants of E. coli," AIChE Natl Mtg., Dallas TX.

Buckelew, M., Wetchuck, J.C., and Ataai, M.M., 1998, "Effect of multiplicity of infection and time of harvest on Production of Viral vectors," AIChE Natl Mtg, Miami Beach, FL.

Chen, Y., Pasquinelli, R., Ataai, M.M., Koepsel, R., Kortes, R.A., and Shepherd, R., 1998, "Study of the Coordination of high affinity six-mer peptide Ser-Pro-His-His-Gly-Gly to a [Pd (II) methyliminodiacetate model of the Cu(II) iminodiacetate chromatography binding site," ACS Natl. Mtg., Boston.

Ozuer, A., Wetchuck, J.C., Russell, B., Glorioso, J.C., and Ataai, M.M., 1998, "Effect of temperature, media Exchange, and post-infection metabolism on infection of HSV1/Vero Cells," AIChE Natl. Mtg., Miami Beach, FL.

Wetchuck, J.C., Glorioso, J.C., and Ataai, M.M., 1998, "Efficient Strategies for High Titer Production of Viral Vectors: HSV-1/Vero Cells as a Case Stud," ACS Natl. Mtg., Boston.

Jesionowski, G., and Ataai, M.M., 1997, "A physiologically designed medium enhances protein productivity of insect cell/reccombinat baculovirus expression system," ACS Natl. Mtg., San Francisco.

Patwardhan, A., Goud, N., Koepsel, R., and Ataai, M.M., 1997, "Selection of optimum affinity tags from a phage displayed peptide library: Application to immobilized metal affinity chromatography," ACS Natl. Mtg., San Francisco.

Patwardhan, A.V., and Ataai, M.M., 1997, "Modeling and simulation of protein purification of a cell extract using immobilized metal affinity chromatography," ACS Natl. Mtg., San Francisco.

Dezura, K., Evans, C., and Ataai, M.M., 1996, "Expression of authentic interleukin 1 receptor antagonist protein: constructing and culturing of a synovial cell carrying a rectoviral integrated gene," AIChE Natl. Mtg., Chicago.

Patwardhan, A.V., and Ataai, M.M., 1996, "Mathematical modeling of protein purification from E. coli cell extract using immobilized metal affinity chromatography: Effect of loading pH on the column capacity and purity of the target protein," AIChE Natl. Mtg., Chicago.

Bhatia, R., Jesionowski, G., and Ataai, M.M., 1995, "Development of a Yeastolate-Free Medium for Insect Cell Cultures," AIChE Natl. Mtg., Miami Beach, FL.

Bhatia, R., Jesionowski, G., and Ataai, M.M., 1995, "Development of New Media for Insect Cell Cultures," Biochemical Eng. Conf., Davos, Switzerland.

Dezura, K., Evans, C., and Ataai, M.M., 1995, "Cultivation of Synovial Cells and Search for Novel Cytokines," ACS Natl. Mtg., Anaheim, CA.

Dezura, K., Evans, C., and Ataai, M.M., 1995, "Cultivation of Synovial Cells and Search for Novel Cytokines," Biochemical Eng. Conf., Davos, Switzerland.

Goel, A., and Ataai, M.M., 1995, "Metabolic insight into Reduction of Acid formation in Bacillus Chemostats," ACS Annual Meeting, Anaheim, CA.

Goel, A., Domach, M.M., and Ataai, M.M., 1995, "Minimization of Acid Formation in Fast Growing Bacterial Cultures," Recombinant DNA Biotechnology Conference, France.

Goud, G., Beckman, E., Koepsel, R., and Ataai, M.M., 1995, "Selection of Short Peptides with High affinity for Copper Using a phage Displayed Peptide Library," ACS Natl. Mtg., Anaheim, CA.

Goud, G., Beckman, E., Koepsel, R., and Ataai, M.M., 1995, "Short Peptides with High Affinity for Various Metal Ions: Strategies and Applications," Biochemical Engineering Conference, Switzerland.

Parwardhan, A.V., and Ataai, M.M., 1995, "Design of Efficient loading and Elution Conditions: Purification of Human Interleukin 1b from E. coli Cell Extract as a Model System," AIChE Natl. Mtg., Miami Beach, FL.

Parwardhan, A.V., and Ataai, M.M., 1995, "Tuning Cell and Target Protein Metal Binding Events for Optimization of Protein recovery Using IMAC," Whitaker Foundation Annual Mtg., Salt Lake City, UT.

Parwardhan, A.V., Zenuzi, M., and Ataai, M.M., 1995, "Simulation and Experimental Studies of IMAC: Identifying the Best Loading and Elution Conditions for Maximum Resolution," AIChE Natl. Mtg., Miami Beach, Fl.

Wagner, W.R., Muzzio, D.J., Rilo, H.R., Deglaus, T., and Ataai, M.M., 1995, "Hepatocyte Culture on Porous Polystyrene Carriers: Response to Substrate, Media, and Shear Environment," AIChE Natl. Mtg., Miami Beach, FL.

Goel, A., and Domach, M.M., 1994, "Metabolic Alterations for High Cellular and Energetic Yields," AIChE, San Francisco.

Goel, A., Domach, M.M., and Ataai, M.M., 1994, "Minimization of Acid Formation in Fast Growing Bacterial Cultures," Recombinant DNA Biotechnology Conference, France.

Beitle, R.R., and Ataai, M.M., 1993, "Optimization of Protein Recovery and Yield Using IMAC," Engineering Foundation Conference on Biochemical Engineering, Princeton, NJ.

Lee, J.W., Domach, M.M., and Ataai, M.M., 1993, "Identification of Metabolic fluxes and Bottleneck in Energetics Through CO2 Regulation," Engineering Foundation Conference on Biochemical Engineering, Princeton, NJ.

Almedia, N., Beckman, E.J., and Ataai, M.M., 1992, "Immobilization of Glucose Oxidase in Thin Polypyrrole Films: Influence of Polymerization Conditions and Film Thickness on Activity and Stability of Immobilized Enzyme," 9th International Biotechnology Symposium, Crystal City, Virginia.

Almedia, N., Beckman, E.J., and Ataai, M.M., 1992, "Immobilization of Glucose Oxidase in Thin Polypyrrole Films: Influence of Polymerization Conditions and Film Thickness on Activity and Stability of Immobilized Enzyme," AIChE Meeting, Miami Beach, FL.

Almedia, N., Becman, E.J., and Ataai, M.M., 1992, "Long Term Activity and Stability of the Immobilized Glucose Oxidase in Thin Polypyrrole Films," AIChE Meeting, Miami Beach, FL.

Beitle, R.R., and Ataai, M.M., 1992, "Highly Efficient Purification of Proteins Extended by a Naturally Occurring Metal-Binding Peptide from Cell Extract," 9th International Biotechnology Symposium, Crystal City, Virginia.

Beitle, R.R., and Ataai, M.M., 1992, "Highly Efficient Purification of Proteins Extended by a Naturally Occurring Metal-Binding Peptide from Cell Extract," AIChE Meeting, Miami Beach, FL.

Beitle, R.R., Sagar, S., Ataai, M.M., and Domach, M.M., 1992, "Metal-Based Affinity Separation of a- and b-Chymotrypsin," AIChE Meeting, Miami Beach, FL.

Beitle, R.R., Sagar, S., Ataai, M.M., and Domach, M.M., 1992, "Metal-Based Affinity Separation of a-and b-Chymotrypsin," 9th International Biotechnology Symposium, Crystal City, Virginia.

Ferrance, J., and Ataai, M.M., 1992, "A Detailed Study of Insect Cell Metabolism," 9th International Biotechnology Symposium, Crystal City, Virginia.

Ferrance, J., and Ataai, M.M., 1992, "A Detailed Study of Insect Cell Metabolism," AIChE Meeting, Miami Beach, FL.

Goel, A., and Ataai, M.M., 1992, "Insights into Effects of Gene Expression on Coordination of Glycolysis and TCA Cycle Reactions," AIChE Meeting, Miami Beach, FL.

Lee, J.W., Domach, M.M., Goel, A., and Ataai, M.M., 1992, "Metabolic Fluxes and Stoichiometries in Wild-Type E. coli and Krebs Cycle Mutants," AIChE Meeting, Miami Beach, FL.

Ataai, M.M., and Goel, A., 1991, "Metabolic Fluxes: Coordination of Glycolysis and TCA Cycle Pathways and Analysis of Energetics and Cellular Yields in B. subtilis," AIChE Meeting, Los Angeles, CA.

Ataai, M.M., Holsinger, M., Goel, A., and Beitle, R.R., 1991, "Sustained High Levels of Protein Production in Vegetative Cultures of B. subtilis," AIChE Meeting, Los Angeles, CA.

Beitle, R.R., Post, A.J., and Ataai, M.M., 1991, "The Penetration of Globular Proteins into Porous Chromatographic Support," AIChE Meeting, Los Angeles, CA.

Beitle, R.R., Sagar, S., Domach, M.M., and Ataai, M.M., 1991, "Mapping of Immobilized Metal- Protein Interactions: Applications to Immobilized Metal Affinity Chromatography," AIChE Meeting, Los Angeles, CA.

Dezura, K., Evans, C., and Ataai, M.M., "Cultivation of Synovial Cells and Search for Novel Cytokines," Biochemical Eng. Conf., Davos, Switzerland.

Jiang, C., Glorioso, J.C., and Ataai, M.M., "Immobilized cobalt affinity purification for HSV-1 gene therapy vector. (invited presentation including about $1800 toward my travel expenses.)," Cell Culture Engineering Conference.

 Publication  Role
 Journal of Biotechnology Progress  Editorial Board